High grade cervical intraepithelial neoplasia positive biopsy: the importance of accurate pre-operative workup.

Background: In cervical cancer screening programs, women with abnormal cytology and confirmation by biopsy are referred for colposcopy for histological evaluation.

Methods: We characterized the presence and the genotype of HPV by Linear Array HPV genotyping assay in cytological samples collected from about 400 women undergoing conization, with reported high CIN grade after biopsy.

Results: The most prevalent genotype was HPV 16, with an increasing presence depending on the severity of the CIN and with the highest incidence in the 26-35 age range.

HPV genotype distribution and age correlation in a selected Italian population undergoing conization.

Background: Detection and genotyping of human papillomavirus (HPV) has gained increasing importance in cervical cancer prevention and treatment of cervical intraepithelial neoplasia (CIN). This study aims to determine the HPV type distribution in cervical specimens obtained from women diagnosed with CIN. We evaluated in a selected Italian population the distribution of HPV genotypes.

Indication of prophylactic vaccines as a tool for secondary prevention in HPV-linked disease.

Purpose: To determine whether quadrivalent HPV vaccination is effective in reducing recurrent disease in women with a previous history of HPV disease.

Methods: All women under 45 years of age treated for HPV-linked disease and with negative HPV test, cytology and colposcopy 3 months after treatment were enrolled. Women were randomly assigned into two groups: a group that received HPV vaccine post treatment and a group that was only submitted to follow-up.

DNA methylation of HOXA10 in eutopic and ectopic endometrium.

Study Question: Does the methylation status of the promoter region of the HOXA10 gene differ in eutopic and ectopic endometrium?

Summary Answer: The eutopic endometrium in women with endometriosis is significantly more methylated when compared with controls.

What Is Known Already: Expression of the HOXA10 gene, which is important for successful implantation, is reduced in women affected by endometriosis.

Study Design, Size And Duration: A pilot study was carried out including 18 women admitted for surgery for endometriosis-related pain (cases) and 12 women admitted for surgery because of non-endometriotic disease (control).