• We tested the hypothesis that biological trait-based plant functional groups provide sufficient differentiation of species to enable generalization about a variety of plant ecophysiological traits or responses to nitrogen (N). • Seedlings of 34 North American grassland and savanna species, representing 5 functional groups, were grown in a glasshouse in an infertile soil with or without N fertilization. • Forbs, C and C grasses, on average, had similar relative growth rates (RGR), followed in declining order by legumes and oaks, but RGR varied greatly among species within functional groups.
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