Tethering of cellulose synthase complex to the plasma membrane relies on the isoform of EXO70A1 in Arabidopsis.

In yeast and mammals, the EXO70 subunit of the exocyst complex plays a key role in mediating the tethering of exocytic vesicles to the plasma membrane (PM). In plants, however, the role of EXO70 in regulating vesicle tethering during exocytosis remains unclear. In land plants, EXO70 has undergone significant evolutionary expansion, resulting in multiple EXO70 paralogues that may allow the exocyst to form various isoforms with specific functions.

Hydroxytyrosol Alleviates Acute Liver Injury by Inhibiting the TNF-α/PI3K/AKT Signaling Pathway via Targeting TNF-α Signaling.

Acute liver injury (ALI) is an injury to liver tissue caused by viruses, drugs, alcohol, and oxygen deprivation, and is one of the most common and serious clinical disorders. Hydroxytyrosol (HT) is a naturally occurring polyphenolic compound isolated from forsythia and has excellent anti-inflammatory properties. However, the effect and mechanisms of HT in ALI remain unclear.

Intestinal stem cell-derived extracellular vesicles ameliorate necrotizing enterocolitis injury.

The therapeutic potential of intestinal stem cell-derived extracellular vesicles (ISCs-EVs) in necrotizing enterocolitis (NEC) remains largely unexplored. This research aims to investigate the therapeutic effects of ISCs-EVs on NEC. Lgr5-positive ISCs were screened from the small intestine of mice by flow cytometry, and ISCs-EVs were isolated by density gradient centrifugation.

Photoperiodic Effect on Growth, Photosynthesis, Mineral Elements, and Metabolome of Tomato Seedlings in a Plant Factory.

The duration of light exposure is a crucial environmental factor that regulates various physiological processes in plants, with optimal timing differing between species and varieties. To assess the effect of photoperiods on the growth and metabolites of a specific truss tomato cultivar, three photoperiods (12 h, 16 h, and 20 h) were tested in a plant factory. Growth parameters, including plant height, stem diameter, fresh and dry weights of shoots and roots, photosynthetic characteristics, mineral content, and metabolome profiles, were analyzed under these conditions.

Pump-free SERS microfluidic chip based on an identification-competition strategy for ultrasensitive and efficient simultaneous detection of liver cancer-related microRNAs.

In this study, we present a pump-free SERS microfluidic chip capable of detecting liver cancer-related miR-21 and miR-155 concurrently with ultra-sensitivity and high efficiency. We employed a FeO@cDNA-AuNPs@Raman reporter@H composite structure and a recognition competition strategy. When the target miRNAs (miR-21 and miR-155) are present in the test liquid, they specifically compete with the nucleic acid complementary strand(H) of FeO@cDNA-AuNPs@Raman reporter@H, causing AuNPs to competitively detach from the surface of FeO, resulting in a decrease in the SERS signal.