Publications by authors named "C Bruce Verchere"
Myeloid cells, including macrophages, neutrophils, dendritic cells, and myeloid-derived suppressor cells, play crucial roles in the innate immune system, contributing to immune defense, tissue homeostasis, and organ development. They have tremendous potential as therapeutic tools for diseases such as cancer and autoimmune disorders, but harnessing cell engineering strategies to enhance potency and expand applications is challenging. Recent advancements in stem cell research have made it possible to differentiate human embryonic stem cells and induce pluripotent stem cells into various cell types, including myeloid cells, offering a promising new approach to generate myeloid cells for cell therapy.
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- This study investigates how the expression of prohormone convertase 1/3 (PC1/3), an enzyme crucial for processing peptide hormones, is affected in islet cells during the progression of type 1 and type 2 diabetes.
- Researchers analyzed pancreatic samples from a diverse group of 54 donors, using immunostaining for detailed examination of PC1/3 in various islet cells at different diabetes stages.
- The results revealed significant changes in islet cell morphology and reduced co-localization of PC1/3 with insulin in type 1 diabetes, while an increase in glucagon and somatostatin in these islets was also noted.
Article Synopsis
- - HumanIslets.com aids diabetes research by providing easy access to islet phenotyping data and analysis tools, available for download.
- - The platform features various data types, including molecular omics, islet function assays, tissue processing metadata, and phenotypes from 547 different donors.
- - As it grows, HumanIslets.com aims to enhance the quality, usability, and accessibility of human islet data for researchers.
Objective: Human embryonic stem cell (hESC; SC)-derived pancreatic β cells can be used to study diabetes pathologies and develop cell replacement therapies. Although current differentiation protocols yield SCβ cells with varying degrees of maturation, these cells still differ from deceased donor human β cells in several respects. We sought to develop a reporter cell line that could be used to dynamically track SCβ cell functional maturation.
View Article and Find Full Text PDFComprehensive molecular and cellular phenotyping of human islets can enable deep mechanistic insights for diabetes research. We established the Human Islet Data Analysis and Sharing (HI-DAS) consortium to advance goals in accessibility, usability, and integration of data from human islets isolated from donors with and without diabetes at the Alberta Diabetes Institute (ADI) IsletCore. Here we introduce HumanIslets.
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