Enhanced proconvulsant sensitivity, not spontaneous rapid swimming activity, is a robust correlate of loss-of-function in stable mutant and F0 crispant hypopigmented zebrafish expressing GCaMP6s.

Zebrafish models of genetic epilepsy benefit from the ability to assess disease-relevant knock-out alleles with numerous tools, including genetically encoded calcium indicators (GECIs) and hypopigmentation alleles to improve visualization. However, there may be unintended effects of these manipulations on the phenotypes under investigation. There is also debate regarding the use of stable loss-of-function (LoF) alleles in zebrafish, due to genetic compensation (GC).

Medical care and initial health status of French children returnees from Jihadist Group Operation Areas: a cross-sectional study from 2017 to 2020 in one referral French hospital.

Introduction: The return of foreign fighters's children whose parents joined the so called « islamic state » in the Iraq-Syrian area, had been a very controversial topic. Since 2017, a national procedure in France has been designed to coordinate their care, including a systematic pediatric medical assessment.

Methods: The aim of this cross-sectional study was to assess the prevalence rate of diseases diagnosed at their arrival in France.

Online integration of capillary electrophoresis and dual detector Taylor dispersion analysis a 3D printed instrument.

Hydrodynamic radius () is a descriptive metric of protein structure with the potential to impact drug development, disease diagnosis, and other important research areas of molecular biology. Common instrumental methods for molecular size characterization are disadvantageous due to high sample consumption, measurements made in non-physiological conditions, and/or inaccurate size determinations. Capillary Taylor dispersion analysis (TDA) is a molecular sizing method that utilizes nL sample volumes and achieves absolute size determination without calibration or comparison to standards.

Inefficient transfer of diatoms through the subpolar Southern Ocean twilight zone.

The Southern Ocean, a region highly vulnerable to climate change, plays a vital role in regulating global nutrient cycles and atmospheric CO via the biological carbon pump. Diatoms, photosynthetically active plankton with dense opal skeletons, are key to this process as their exoskeletons are thought to enhance the transfer of particulate organic carbon to depth, positioning them as major vectors of carbon storage. Yet conflicting observations obscure the mechanistic link between diatoms, opal and particulate organic carbon fluxes, especially in the twilight zone where greatest flux losses occur.

Reflections on a Dobutamine Shortage in an Academic Health System: A Roadmap for Risk Reduction.

In a landscape of increasingly frequent and severe drug shortages, this article describes an interdisciplinary strategy for managing a nationwide shortage of dobutamine in an academic health system. The authors outline an approach that centers on leveraging information technology resources, minimizing waste, conserving supply, and centralizing supply. These efforts, which enabled the organization to consistently supply dobutamine to those patients who needed it most, could form a model for health systems to follow during future drug shortages.