Publications by authors named "C Bonzano"
Article Synopsis
- The study aimed to assess the diversity of microbial keratitis by measuring species richness and the Shannon Diversity Index using corneal impression membranes for sample collection.
- Samples were analyzed by Biolab Laboratory in Italy, with DNA extraction and sequencing performed; low-quality sequences were filtered out before employing Kraken2 for microbial community analysis.
- Results revealed a diverse range of over 800 species in some samples, with richness values between 200 to 600 in most, highlighting the complexity of bacterial phyla involved in keratitis infections.
In Vivo Confocal Microscopy Characterization of Candida parapsilosis Keratitis.
Eye Contact Lens
February 2024
The present clinical case concerns two patients with mycotic keratitis because of Candida parapsilosis in which corneal confocal microscopy presented a characteristic feature of this pathogen. Both described patients used a therapeutic contact lens and administered a therapy with steroid eye drops which are well known predisposing factors for the onset of corneal mycoses. This report can be useful for correctly identifying the pathologic condition and quickly directing the therapy.
View Article and Find Full Text PDFIntroduction: The purpose of this short article is to report the clinical outcomes of topical 0.1% ciclosporin cationic emulsion (CsA-CE) used on label in children with vernal keratoconjunctivitis (VKC).
Methods: In this prospective, non-comparative, observational study children affected by active severe VKC were treated for at least 12 months with topical 0.
Purpose: To evaluate the microbiota of culture negative Corneal Impression Membrane (CIM) microbial keratitis samples with the use of shotgun metagenomics analysis.
Methods: DNA of microbial keratitis samples were collected with CIM and extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre). DNA was fragmented by sonication into fragments of 300 to 400 base pairs (bp) using Bioruptor® (Diagenode, Belgium) and then used as a template for library preparation.
Nail-patella syndrome (NPS) is a rare autosomal dominant disease characterized by nail dysplasia, aplastic or hypoplastic patellae, elbow dysplasia, and presence of iliac horns. Renal or ocular abnormalities are also associated with the disease. We report the case of a 57-year-old woman affected by NPS and having haploinsufficiency of the LMX1B gene who experienced severe bilateral chronic angle-closure glaucoma in both eyes and that was successfully managed with a flap-express procedure in the right eye.
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