A global genetic interaction network by single-cell imaging and machine learning.

Cellular and organismal phenotypes are controlled by complex gene regulatory networks. However, reference maps of gene function are still scarce across different organisms. Here, we generated synthetic genetic interaction and cell morphology profiles of more than 6,800 genes in cultured Drosophila cells.

Time-resolved mapping of genetic interactions to model rewiring of signaling pathways.

Unlabelled: Context-dependent changes in genetic interactions are an important feature of cellular pathways and their varying responses under different environmental conditions. However, methodological frameworks to investigate the plasticity of genetic interaction networks over time or in response to external stresses are largely lacking. To analyze the plasticity of genetic interactions, we performed a combinatorial RNAi screen in cells at multiple time points and after pharmacological inhibition of Ras signaling activity.

Bait Preference of Free-Ranging Feral Swine for Delivery of a Novel Toxicant.

Invasive feral swine (Sus scrofa) cause extensive damage to agricultural and wildlife resources throughout the United States. Development of sodium nitrite as a new, orally delivered toxicant is underway to provide an additional tool to curtail growth and expansion of feral swine populations. A micro-encapsulation coating around sodium nitrite is used to minimize detection by feral swine and maximize stability for the reactive molecule.

Deer response to exclusion from stored cattle feed in Michigan, USA.

Disease and damage from white-tailed deer (Odocoileus virginianus) continually threaten the livelihood of agricultural producers and the economy in the United States, as well as challenge state and federal wildlife managers. Threats can be partially addressed by excluding free-ranging deer from livestock-related resources. Throughout the year, use of stored livestock feed by deer in northern Lower Michigan (MI), USA fluctuates, though their presence is relatively consistent.

A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila.

Innate immune signalling pathways are evolutionarily conserved between invertebrates and vertebrates. The analysis of NF-kappaB signalling in Drosophila has contributed important insights into how organisms respond to infection. Nevertheless, significant gaps remain in our understanding of how the activation of intracellular signalling elicits specific transcriptional programs.