Specific cultivation-independent enumeration of viable cells in probiotic products using a combination of fluorescence hybridization and flow cytometry.

This study introduces an optimized integration of flow cytometry and fluorescence hybridization (Flow-FISH) as an approach for the specific enumeration of gram-positive bacteria in probiotic products, overcoming the limitations of conventional methods. The enhanced Flow-FISH technique synergizes the rapid and automated capabilities of flow cytometry with the high specificity of FISH, facilitating the differentiation of viable cells at the species level within probiotic blends. By analyzing lyophilized samples of , , and subsp.

Symbioflor2 Genotypes Enhance Ileal and Colonic Gene Expression Associated with Mucosal Defense in Gnotobiotic Mice.

Symbioflor2 is a probiotic product composed of six genotypes, which has a beneficial effect on irritable bowel syndrome. Our objective was to understand the individual impact of each of the six genotypes on the host, together with the combined impact of the six in the compound Symbioflor2. Gnotobiotic mice were mono-associated with one of the six genotypes or associated with the compound product.

Evaluation of the applicability of the aquatic ascomycete sp. UHH 5-1-03 for the removal of pharmaceutically active compounds from municipal wastewaters using membrane bioreactors.

Membrane bioreactors (MBRs) augmented with terrestrial white-rot basidiomycetes have already been tested for the removal of pharmaceutically active compounds (PhACs) from wastewaters. Within the present study, an aquatic ascomycete ( sp.) was initially demonstrated to efficiently remove several PhACs at their real environmental trace concentrations from nonsterile municipal wastewater on a laboratory scale.

A Review of Research Conducted with Probiotic Marketed as Symbioflor.

This review article summarizes the scientific literature that is currently available about a probiotic that is known under the name Symbioflor . The probiotic is marketed for human use and has been subjected to over 20 years of scientific research. As is presented here, the available literature not only contains multiple works to investigate and analyse the probiotic activity of this , but also describes a variety of other research experiments, dealing with a surprising and interesting range of subjects.

Virulence genes in a probiotic E. coli product with a recorded long history of safe use.

The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E.