Publications by authors named "C A Moxon"
Postmortem single-cell studies have transformed understanding of lower respiratory tract diseases (LRTDs), including coronavirus disease 2019 (COVID-19), but there are minimal data from African settings where HIV, malaria and other environmental exposures may affect disease pathobiology and treatment targets. In this study, we used histology and high-dimensional imaging to characterize fatal lung disease in Malawian adults with (n = 9) and without (n = 7) COVID-19, and we generated single-cell transcriptomics data from lung, blood and nasal cells. Data integration with other cohorts showed a conserved COVID-19 histopathological signature, driven by contrasting immune and inflammatory mechanisms: in US, European and Asian cohorts, by type I/III interferon (IFN) responses, particularly in blood-derived monocytes, and in the Malawian cohort, by response to IFN-γ in lung-resident macrophages.
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- The study aimed to investigate liver histopathology in children who died of acute illness in Malawi, specifically looking at nutritional status and its effects on liver mitochondria and peroxisomes.
- Researchers collected liver tissue from eleven children under five, categorizing them into non-wasted, severely wasted, and edematous malnutrition groups to analyze histological differences using advanced microscopy techniques.
- Results showed that children with edematous malnutrition had significantly fewer and more abnormal mitochondria compared to the other groups, indicating that targeting liver metabolic functions could help improve outcomes for children suffering from severe malnutrition.
COVID-19 is characterized by a broad range of symptoms and disease trajectories. Understanding the correlation between clinical biomarkers and lung pathology during acute COVID-19 is necessary to understand its diverse pathogenesis and inform more effective treatments. Here, we present an integrated analysis of longitudinal clinical parameters, peripheral blood markers, and lung pathology in 142 Brazilian patients hospitalized with COVID-19.
View Article and Find Full Text PDFBackground: Adenoviral vector-based COVID-19 vaccine-induced immune thrombotic thrombocytopenia (VITT) is rare but carries significant risks of mortality and long-term morbidity. The underlying pathophysiology of severe disease is still not fully understood. The objectives were to explore the pathophysiological profile and examine for clinically informative biomarkers in patients with severe VITT.
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- The chapter discusses methods to study how parasitized red blood cells interact with endothelial cells in malaria patients using skin and subcutaneous tissue biopsies.
- It highlights different biopsy techniques like punch and needle biopsies for collecting tissue samples and the use of smears for immunofluorescence staining.
- Additionally, it explores how the dissected endothelial cells can be isolated for further laboratory experiments, including culture, immunophenotyping, and advanced microscopy techniques.