Publications by authors named "C A Mayhew"
Article Synopsis
- The study focuses on tissue engineering aimed at repairing the small bowel using human intestinal organoids (HIOs) created from human pluripotent stem cells.
- Researchers tested the ability of these organoids to engraft and aid healing in a rodent model with acute bowel damage, showing they can proliferate and integrate into various layers of the intestine.
- Key results included the restoration of the mucosal layer, integration into muscle and blood vessel tissues, and the long-term presence of diverse cell types, highlighting the role of mesenchyme in effective intestinal repair.
Transcription factor (TF) gene knockout or knockdown experiments provide comprehensive downstream effects on gene regulation. However, distinguishing primary direct effects from secondary effects remains challenging. To assess the direct effect of TF binding events, we present a protocol for establishing a doxycycline (Dox)-inducible CRISPRd system in human pluripotent stem cells (hPSCs).
View Article and Find Full Text PDFInducible loss-of-function strategies are crucial for understanding gene function. However, creating inducible, multiple-gene knockout models is challenging and time-consuming. Here, we present a protocol for establishing a doxycycline-inducible CRISPR interference (CRISPRi) system to concurrently silence multiple genes in human induced pluripotent stem cells (hPSCs).
View Article and Find Full Text PDFCurrent microbiological methods for pneumonia diagnosis require invasive specimen collection and time-consuming analytical procedures. There is a need for less invasive and faster methods to detect lower respiratory tract infections. The analysis of volatile metabolites excreted by pathogenic microorganisms provides the basis for developing such a method.
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