Publications by authors named "C A Alter"
Lipid nanoparticles (LNPs) have emerged as the leading nonviral nucleic acid (NA) delivery system, gaining widespread attention for their use in COVID-19 vaccines. They are recognized for their efficient NA encapsulation, modifiability, and scalable production. However, LNPs face efficacy and potency limitations due to suboptimal intracellular processing, with endosomal escape efficiencies (ESE) below 2.
View Article and Find Full Text PDFHistological analysis with 2,3,5-triphenyltetrazolium chloride (TTC) staining is the most frequently used tool to detect myocardial ischemia/reperfusion injury. However, its practicality is often challenged by poor image quality in gross histology, leading to an equivocal infarct-boundary delineation and potentially compromised measurement accuracy. Here, we introduce several crucial refinements in staining protocol and sample processing, which enable TTC images to be analyzed with light microscopy.
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- High salt (HS) intake can worsen immune responses and contribute to the development of hypertensive vascular diseases, particularly after exposure to Ang II, a hormone that raises blood pressure.
- In experiments with mice, a short period of HS consumption led to increased inflammation and a higher occurrence of severe vascular issues when combined with Ang II infusion, despite no blood pressure differences between groups.
- The study concludes that transient HS intake triggers a mild immune response that becomes problematic when followed by Ang II exposure, suggesting that HS acts as a precursor to more significant hypertension-related health risks.
Lipid nanoparticles (LNPs) are successfully used for RNA-based gene delivery. In the context of gene replacement therapies, however, delivery of DNA expression plasmids using LNPs as a non-viral vector could be a promising strategy for the induction of longer-lasting effects. Therefore, DNA expression plasmids (3 to 4 kbp) coding for fluorescent markers or luciferase were combined with LNPs.
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