Publications by authors named "Byung-Lak Lim"
An antioxidant substance (AOS) obtained from an enzymatic extract of the red seaweed Gloiopeltis tenax was purified by DEAE-Sephadex CL-6B and Sephadex G-100 column chromatography. The purification yield of AOS was 14.3%.
View Article and Find Full Text PDFThe biochemical mechanisms of Chlorella vulgaris protection against cadmium (Cd)-induced liver toxicity were investigated in male Sprague-Dawley rats (5 weeks of age, weighing 90-110 g). Forty rats were randomly divided into one control and three groups treated with 10 ppm Cd: one Cd without Chlorella (Cd-0C), one Cd with 5% Chlorella (Cd-5C), and one Cd with 10% Chlorella (Cd-10C) groups. The rats had free access to water and diet for 8 weeks.
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- A mannanase enzyme was purified from genetically modified E. coli that contained a mannanase gene from Bacillus subtilis, with a molecular mass of 38 kDa and optimal activity at pH 6.0 and 60°C.
- The enzyme displayed high specific activity on locust bean gum, encountered slight inhibition from certain ions, and was enhanced by Fe(2+), though it lost activity when stored with Fe(2+) at low temperatures.
- It effectively hydrolyzed various galactomannans and xylans, producing oligosaccharides like mannose and mannotetraose, but showed no activity towards carboxymethylcellulose and specific synthetic substrates.
Cloning and strong expression of a Bacillus subtilis WL-3 mannanase gene in B. subtilis.
J Microbiol Biotechnol
October 2007
A gene encoding the mannanase of Bacillus subtilis WL-3, which had been isolated from Korean soybean paste, was cloned into Escherichia coli and the nucleotide sequence of a 2.7-kb DNA fragment containing the mannanase gene was subsequently determined. The mannanase gene, designated manA, consisted of 1,080 nucleotides encoding polypeptide of 360 amino acid residues.
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