Comparative Analysis of Humoral Immune Response and Cognate Antigen Detection in Experimentally Infected Sprague Dawley Rats with Biotype 1.

This study investigated the IgG-specific humoral immune responses against specific antigen-like whole-cell antigen (WCA), outer membrane protein (OMP), periplasmic protein (PP), and cytoplasmic protein (CP) during the acute and subacute stages of biotype 1 infection in Sprague Dawley (SD) rats. The intraperitoneal method was used to experimentally infect forty-four 6- to 8-week-old SD rats with 1 × 10 colony-forming units (CFUs) of biotype 1. Following inoculation, the rat was serially sampled for serum at 0, 3, 7, 14, 21, 28, 35, 42, 60, 90, and 120 days.

and IFN-γ and IL-10 Measurement in Experimental Biotype 1 Infection in Sprague-Dawley Rats.

The immune response to mainly depends on antigen-specific T cell activation, CD4 and CD8 T cells, and specific humoral response. Protective immune response against infection has not been performed in the Sprague-Dawley (SD) rat model. We measured bacterial kinetics in addition to and interferon gamma (IFN-γ) and interleukin-10 (IL-10) production against crude protein in the SD rats at different days of postinfection with biotype 1 by indirect enzyme-linked immunosorbent assay.

The Profile of Immunoglobulin A and Immunoglobulin G Subclasses in Sprague Dawley Rats Experimentally Infected with Biotype 1.

This study measured total serum immunoglobulin A (IgA), immunoglobulin G (IgG)1, IgG2a response against whole cell antigen (WCA), outer membrane protein (OMP), periplasmic protein (PP), cytoplasmic protein (CP), and crude protein (CBP) of in experimental brucellosis induced with biotype 1 in Sprague Dawley (SD) rats during a 17-week infection period. Six- to 8-week-old SD rats ( = 44) were experimentally infected with 1 × 10 colony forming unit of biotype 1 through the intraperitoneal route. Serial serum samples were collected from the rat at 0, 3, 7, 14, 21, 28, 35, 42, 60, 90, and 120 days after inoculation.

Characteristics of the immune response during acute brucellosis in Sprague-Dawley rats.

Background: Brucella is a facultative, intracellular pathogen that causes severe disease in animals and humans. Immunity against Brucella involves both humoral and cellular responses. To investigate the characteristics of immune response in acute brucellosis in Sprague-Dawley (SD) rats, IgG and its subclass specific immunoglobulins' (IgG1 and IgG2a) response in sera against B.

Effects of Brucella abortus biotype 1 infection on the reproductive performance of Sprague-Dawley rats.

The aim of this study was to assess the impact of Brucella abortus biotype 1 infection on the reproductive performance using Sprague-Dawley (SD) rat model. Virgin female SD rats (n = 24) were infected intraperitoneally with 0.1 mL of saline containing 1 x 10(11) Colony Forming Unit (CFU) of pathogenic B.

Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay.

Background: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay.

Methodology: Female SD rats were orally administered with 1.0 x 10(7) colony forming unit (cfu) suspension of SRB51 and half of these SD rats were challenged at 4 weeks post inoculation with 1.

Evaluation of the Sprague-Dawley rat as a model for vertical transmission of Brucella abortus.

Vertical transmission of Brucella abortus in Sprague-Dawley (SD) rats was verified with microbiologic, serologic, and polymerase chain reaction (PCR) methods. The 38 initially Brucella-free SD rats, weighing 200 to 250 g, were injected subcutaneously with 50 microL of a suspension containing 1 x 10(9) colony-forming units (cfu) of B. abortus biotype 1 Korean isolate.

Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows.

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses.

[Fractionation of antigen for ELISA of bovine fascioliasis].

In order to obtain the most specific and sensitive antigen from crude antigens of Fasciola hepatica for the immunodiagnosis of bovine fascioliasis by the enzyme linked immunosorbent assay(ELISA), phosphate buffered saline extract of F. hepatica was prepared. The crude extract was fractionated into 7 antigens using Sephadex G-100 column chromatography.

[Application of micro-ELISA in serodiagnosis of fascioliasis in cattle].

Fascioliasis in cattle is one of the most common and very serious trematode diseases in Korea. In the present study, the enzyme linked immunosorbent assay (ELISA) was applied in the diagnosis of fascioliasis using antigen of Fasciola hepatica, peroxidase of conjugate anti-cattle IgG and orthophenylenediamine as a substrate by micro-method technique of Voller et al. (1976b) and MacLaren (1978) with a slight modification.

[Experimental studies on the second intermediate hosts of Clonorchis sinensis IV. Observations on the fate of metacercariae of Clonorchis sinensis in the fish host, Cultriculus eigenmanni].

In order to judge appropriately the suitability as the second intermediate host of Clonorchis sinensis, the present experiments were proposed to survey the metacercaria of C. sinensis in the fish host, Cultriculus eigenmanni, in the field, and to observe, by experimental infection of the fish with Clonorchis cercaria, the penetrating ability of the cercaria, maturity, the process of degeneration and extinction, and infectivity of the metacercaria. The following is a brief summary of the leading facts gained through the experiments.

[Survey On Encysted Cercaria Of Trematodes From Fresh-Water Fishes In Tongjin Riverside Areas In Korea].

In an attempt to clarify the epidemiological feature of distomiasis in Tongjin riverside area, the prevalence of distomiasis in the residents and infection rates of the metacercariae in fresh-water fishes were investigated at the upper, middle and lower reaches of the river from January to April 1984. The results obtained were summarized as follows: Out of a total of 931 fresh-water fishes which composed of 33 different species, 611 fishes (65.6 %) of 31 species were found positive with digenetic trematode metacercariae of 16 different species, and there were some differences in infection rates of the metacercariae among the fishes in the 3 parts of the river; 53.

[Survey On Encysted Cercariae Of Trematodes From Fresh-Water Fishes In Mangyeong Riverside Area].

In order to clarify infestation pattern for the encysted larvae of digenetic trematodes from fresh-water fishes, this survey was carried out from March to September, 1983. A total of 380 fishes of 32 species were collected with netting at the three reaches, upper, middle and lower in Mangyeong riverside area. After the fishes were dissected into small scraps, they were pressed under cover glass and examined for the presence of those of digenetic trematodes with a microscope.

[Epidemiological Studies Of Clonorchis Sinensis In Mangyeong Riverside Areas In Korea].

In an attempt to clarify the epidemiological feature of C. sinensis in Mangyeong riverside area, the prevalence of clonorchiasis, infestation rate of the cercariae in Parafossarulus manchouricus, and detection rate of the metacercariae in fresh-water fishes were investigated from March 1 to September 30, 1983 at the upper, middle and lower reaches of the river. The results obtained were summarized as follows:Detection rate of C.

[The Wormicidal Substance Of Fresh Water Fishes On Clonorchis Sinensis: Iv. Preliminary Research On The Wormicidal Substance From Mucus Of Cyprinus Carpio Nudus].

As a series of studies on the suitability as a second intermediate host of Clonorchis sinensis, the wormicidal effects of mucus from epidermis of Cyprinus carpio nudus were observed. The results obtained were summarized as follows: On the silica gel thin layer chromatography with the greenish yellow supernatant in acetone:benzene/10 : 90 as carrier, seven spots were observed in iodine chamber. The spot of Rf.

[Experimental Infection Of Clonorchis Sinensis To Cyprinus Carpio Nudus].

As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nudus. The results obtained were summarized as follows: 1. The cercariae which attempted to contact with Cyprinus carpio nudus in the water were observed under the stereomicroscope.

[Isolation Of Anthelmintic Substance On Clonorchis Sinensis From Tuber Of Bletilla Striata].

The present work was done to isolate the anthelmintic substance from the tuber of Bletilla striata which grows Gangweon-do of the Korea peninsula. The dried B. striata tuber was extracted with ethyl ether by a Soxhlet extractor at 40 degrees C for 24 hours, and then the extract was separated into insoluble material (negative wormicidal) and soluble material (positive wormicidal) by petroleum ether solvent.

[Localization and isolation of anthelmintic activity on Clonorchis sinensis from stem cortex of Machilis thunbergii].

The present work was to localize and isolate the anthelmintic substance from stem cortex of Machilis thunbergii which grows in all parts of the Korea peninsula. The dried M. thunbergii bark was extracted with a solvent mixture (Methanol: Ethyl acetate/1:1) by a Soxhlet extractor at 70 degrees C for 24 hours, and then the extract was separated into solid matter (negative wormicidal) and benzene extract (positive wormicidal) by benzene solvent.

[The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: III. Seasonal variation on the wormicidal substances of mucous substances of Carassius carassius].

The epidermal mucous substances of C. carassius which were extracted by ether were separated into various spots on the silica gel thin layer chromatography and separated into various fractions in the silica gel column chromatography, using petroleum ether (30 %) and chloroform(70 %) as a solvent. The wormicidal effects of the spots and the fractions on the cercaria, the liberating metacercaria and the adult of C.

[The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes].

The present work which was investigated in July 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (Cyprinus carpio, Parasilurus asotus, Anguilla japonica, Ophicephalus argus, Carassius carassius; golden crusian carp, Misgurnus anguillicaudatus, Zacco platypus, Pseudorasbora parva and Carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of Clonorchis sinensis. The mucous substances extracted by ether from the above 9 species of fishes were separated into many spots on the silica gel thin layer chromatography, and divided into many fractions in the silica gel column chromatography which used petroleum ether (30%) and chloroform (70%) as a solvent. 1.

[The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: I Preliminary Research On The Wormicidal Substance From Mucous Substance Of Carassius Carassius].

The present work was to observe the wormicidal effects of the external mucous substance of Carassius carassius and Pseudorasbora parva on cercaria, metacercaria (liberating) and adult of Clonorchis sinensis. The mucous substance which was extracted by ether was separated into various spots on the silica gel thin layer chromatography and separated into various fractions in the silica gel column chromatography, using petroleum ether(30%) and chloroform(70%) as solvent. 1.