Draft genome sequence of species strain 25GB23B1, cultivated from arctic surface seawater.

We report a draft genome sequence for species strain 25GB23B1, isolated from arctic surface seawater off the coast of Alaska. The whole genome sequence will provide knowledge of the bacteria's relationship to its environment and possibly a new species of .

AP-1 Mediates Cellular Adaptation and Memory Formation During Therapy Resistance.

Cellular responses to environmental stimuli are typically thought to be governed by genetically encoded programs. We demonstrate that melanoma cells can form and maintain cellular memories during the acquisition of therapy resistance that exhibit characteristics of cellular learning and are dependent on the transcription factor AP-1. We show that cells exposed to a low dose of therapy adapt to become resistant to a high dose, demonstrating that resistance was not purely selective.

Positive public attitudes towards agricultural robots.

Robot technologies could lead to radical changes in farming. But what does the public know and think about agricultural robots? Recent experience with other agricultural technologies-such as plant genetic engineering-shows that public perceptions can influence the pace and direction of innovation, so understanding perceptions and how they are formed is important. Here, we use representative data from an online survey (n = 2269) to analyze public attitudes towards crop farming robots in Germany-a country where new farming technologies are sometimes seen with skepticism.

Personalized Deep Learning for Substance Use in Hawaii: Protocol for a Passive Sensing and Ecological Momentary Assessment Study.

Background: Artificial intelligence (AI)-powered digital therapies that detect methamphetamine cravings via consumer devices have the potential to reduce health care disparities by providing remote and accessible care solutions to communities with limited care solutions, such as Native Hawaiian, Filipino, and Pacific Islander communities. However, Native Hawaiian, Filipino, and Pacific Islander communities are understudied with respect to digital therapeutics and AI health sensing despite using technology at the same rates as other racial groups.

Objective: In this study, we aimed to understand the feasibility of continuous remote digital monitoring and ecological momentary assessments in Native Hawaiian, Filipino, and Pacific Islander communities in Hawaii by curating a novel data set of longitudinal Fitbit (Fitbit Inc) biosignals with the corresponding craving and substance use labels.

Review: Ethical responsibilities and transformation strategies of focal companies in the meat supply chain: the implementation dilemma.

In Germany (and other Organisation for Economic Co-operation and Development countries), the need for a fundamental transformation of livestock farming has become increasingly evident in recent years. Two broad-based stakeholder commissions including the German farmers' association, the meat industry, and Non-Governmental Organizations have endorsed this demand. Nevertheless, major steps towards its implementation are still missing - a circumstance we refer to in this paper as the implementation dilemma.

Diverse clonal fates emerge upon drug treatment of homogeneous cancer cells.

Even among genetically identical cancer cells, resistance to therapy frequently emerges from a small subset of those cells. Molecular differences in rare individual cells in the initial population enable certain cells to become resistant to therapy; however, comparatively little is known about the variability in the resistance outcomes. Here we develop and apply FateMap, a framework that combines DNA barcoding with single-cell RNA sequencing, to reveal the fates of hundreds of thousands of clones exposed to anti-cancer therapies.

Validation of a form for assessing the professional performance of residents in cardiology by nurses.

Background: There is a strong need for transformation in our assessment systems from one that evaluates performance based on levels of training to another that focuses on professional competence to meet the expected requirements for the practice of the profession. The aim of this study is to validate for the first time a Spanish version of a new tool for assessing the professional performance of residents by nurses newly developed in the Ottawa Hospital (O-RON).

Materials And Methods: After the author's written authorization, the original O-RON form was translated and cross-culturally adapted.

Systematically analysing the acceptability of pig farming systems with different animal welfare levels when considering intra-sustainability trade-offs: Are citizens willing to compromise?

In recent years, intensive pig husbandry has been subject to increasing public criticism, including a clear demand for more animal-friendly housing systems in many countries. However, such systems are associated with trade-offs at the expense of other sustainability domains, which challenges implementation and makes prioritization necessary. Overall, research is scarce that systematically analyses citizens' evaluation of different pig housing systems and associated trade-offs.

Neurofilament light chain serum levels correlate with the severity of neurotoxicity after CAR T-cell treatment.

Antitumor therapy with CD19-targeted chimeric antigen receptor (CAR) modified T cells is highly efficient. However, treatment is often complicated by a unique profile of unpredictable neurotoxic adverse effects of varying degrees known as immune effector cell-associated neurotoxicity syndrome (ICANS). We examined 96 patients receiving CAR T cells for refractory B-cell malignancies at 2 major CAR T-cell treatment centers to determine whether serum levels of neurofilament light chain (NfL), a marker of neuroaxonal injury, correlate with the severity of ICANS.

Virtual farm tours-Virtual reality glasses and tablets are suitable tools to provide insights into pig husbandry.

Apart from improving husbandry conditions and animal welfare, there is a clear public demand to increase transparency in agricultural activities. Personal farm tours have shown to be appreciated by citizens but are limited in their impact because of hygiene requirements and accessibility. Virtual farm tours are a promising approach to overcome these limitations but evidence on their perceptions is missing.

Outcomes of protracted bacterial bronchitis in children: A 5-year prospective cohort study.

Background And Objective: Long-term data on children with PBB has been identified as a research priority. We describe the 5-year outcomes for children with PBB to ascertain the presence of chronic respiratory disease (bronchiectasis, recurrent PBB and asthma) and identify the risk factors for these.

Methods: Prospective cohort study was undertaken at the Queensland Children's Hospital, Brisbane, Australia, of 166 children with PBB and 28 controls (undergoing bronchoscopy for symptoms other than chronic wet cough).

Health-resource use and quality of life in children with bronchiectasis: a multi-center pilot cohort study.

Background: Bronchiectasis in children is an important, but under-researched, chronic pulmonary disorder that has negative impacts on health-related quality of life. Despite this, it does not receive the same attention as other chronic pulmonary conditions in children such as cystic fibrosis. We measured health resource use and health-related quality of life over a 12-month period in children with bronchiectasis.

A quantitative, retrospective inquiry of the impact of a provider-guided low-carbohydrate, high-fat diet on adults in a wellness clinic setting.

Aims: Despite the critical status of obesity as an epidemic chronic illness in the United States contributing toward diabetic and cardiovascular disease as well as early preventable death, treatment approaches in primary care remain in conflict; providers lack evidence-based guidelines toward impactful disease management, particularly from dietary approaches. This study aimed to evaluate the impact of initiation of a 5-10% low-carbohydrate, 75-85% high-fat diet on specific clinical indicators of obesity, a metabolic disease associated with increased morbidity and mortality, at baseline and six months in an adult population.

Materials And Methods: Utilizing a retrospective electronic medical record data collection protocol, one hundred patients with obesity in a wellness clinic in the Southwestern United States between 2017 and 2018 prescribed a low-carbohydrate, high-fat diet were selected via simple random sampling.

Does picture background matter? People's evaluation of pigs in different farm settings.

Pictures of farm animals and their husbandry systems are frequently presented in the media and are mostly connected to discussions surrounding farm animal welfare. How such pictures are perceived by the broader public is not fully understood thus far. It is presumable that the animals' expressions and body languages as well as their depicted environment or husbandry systems affect public perception.

Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus.

The Australian paralysis tick (Ixodes holocyclus) secretes neuropathic toxins into saliva that induce host paralysis. Salivary glands and viscera were dissected from fully engorged female I. holocyclus ticks collected from dogs and cats with paralysis symptoms.

American and German attitudes towards cow-calf separation on dairy farms.

Public concerns regarding the quality of life of farm animals are often focused on specific practices such as separating the cow and calf immediately after birth. The available scientific literature provides some evidence in support of this practice (including reduced acute responses to separation when it does occur), as well as evidence of disadvantages (such as increased risk of uterine disease in cows). The aim of this study is to systematically examine public views around this practice.

Fast Growing Plantations for Wood Production - Integration of Ecological Effects and Economic Perspectives.

Biomass crops are perceived as a feasible means to substitute sizeable amounts of fossil fuel in the future. A prospect of CO2 reduction (resp. CO2 neutrality) is credited to biomass fuels, and thus a potential contribution to mitigate climate change.