A randomised controlled trial to evaluate the efficacy of a multi-step oral hygiene product intervention to improve oral health in partial denture wearers.

Objectives: This study was designed to evaluate the effectiveness of a multi-step oral hygiene intervention among removal partial denture (RPD) wearers.

Methods: A 12-week, single-blind, randomised, 2-armed, parallel-group, controlled trial was conducted with 49 RPD-wearing participants with mild-moderate gingivitis. The intervention group received stannous fluoride toothpaste, sodium fluoride mouth rinse, and an antibacterial denture cleanser foam.

A Randomized Clinical Study Investigating the Stain Removal Efficacy of Two Experimental Dentifrices.

Objective: This study aims to evaluate extrinsic tooth stain removal and whitening efficacy of two experimental dentifrices containing (i) 5% sodium tripolyphosphate (STP)/1% micronized alumina or (ii) 5% STP/1% micronized alumina with abrasive silica (ED2) compared to a regular fluoride dentifrice (RFD) following 8 weeks of use.

Materials And Methods: This was a single-center, randomized, controlled, blind, three-arm, stratified, parallel-group study. Eligible participants underwent clinical assessment of stain on the facial/lingual surfaces of maxillary and mandibular teeth using the modified Lobene stain index (MLSI), and shade of the facial surfaces of the central and lateral maxillary incisors using the VITA Bleachedguide 3D-Master (VITA) shade guide.

A randomized clinical study to assess the performance of a marketed denture adhesive in a model of food infiltration in healthy, edentulous adults.

Objectives: An optimized food infiltration methodology was utilized to assess the objective and subjective efficacy of a marketed denture adhesive regarding denture dislodgment and infiltration and perception of food particles under maxillary and mandibular dentures. A pilot study helped optimize methodologies before the efficacy study.

Materials And Methods: Participants were healthy adults (n =48 for both studies) with fair- to well-fitting and well-made full maxillary and mandibular dentures.

Interdental and subgingival microbiota may affect the tongue microbial ecology and oral malodour in health, gingivitis and periodontitis.

Background And Objective: Oral malodour is often observed in gingivitis and chronic periodontitis patients, and the tongue microbiota is thought to play a major role in malodorous gas production, including volatile sulphur compounds (VSCs) such as hydrogen sulphide (H S) and methanethiol (CH SH). This study aimed to examine the link between the presence of VSCs in mouth air (as a marker of oral malodour) and the oral bacterial ecology in the tongue and periodontal niches of healthy, gingivitis and periodontitis patients.

Methods: Participants were clinically assessed using plaque index, bleeding on probing (BOP) and periodontal probing depths, and VSC concentrations in their oral cavity measured using a portable gas chromatograph.

Efficacy of an Experimental Occlusion Technology Toothpaste in the Relief of Dentinal Hypersensitivity: An 8-week Randomised Controlled Trial.

Purpose: A novel anhydrous toothpaste formulation has been developed containing the anti-dentinal hypersensitivity (DH) ingredient stannous fluoride (SnF2).

Materials And Methods: This randomised, controlled, examiner-blind, parallel-group, stratified (by baseline Schiff sensitivity score) study compared efficacy of an experimental 'Test' toothpaste (n = 67) containing 0.454% SnF2, 0.

In situ efficacy of an experimental toothpaste on enamel rehardening and prevention of demineralisation: a randomised, controlled trial.

Background: A novel sodium fluoride toothpaste containing lactate ion and polyvinylmethylether-maleic anhydride has been developed to promote enamel remineralisation and resistance to demineralisation. In this in situ study, we compared this toothpaste ('Test') with a stannous fluoride-zinc citrate (SnF-Zn) toothpaste ('Reference') (both 1100-1150 ppm fluoride) and a fluoride-free toothpaste ('Placebo') using an enamel dental erosion-rehardening model.

Methods: In each phase of this randomised, investigator-blind, crossover study, participants wore palatal appliances holding bovine enamel specimens with erosive lesions.

Impact of toothpaste on oral health-related quality of life in people with dentine hypersensitivity.

Background: Dentine hypersensitivity can impact functional status and everyday activities such as eating and talking. This study aimed to assess changes in oral health-related quality of life measures in individuals with dentine hypersensitivity following long-term use (24 weeks) of a commercially available toothpaste marketed for dentine hypersensitivity relief.

Methods: This study was conducted across two sites and enrolled 75 adults with ≥2 non-adjacent sensitive teeth.

A randomized clinical study to evaluate the effect of denture adhesive application technique on food particle accumulation under dentures.

Food ingress under dentures is a common problem that may be reduced by denture adhesive use. The objective of this study was to explore the effect of the mode of application of a denture adhesive on reducing accumulation of food particles under dentures. This was a single-centre, controlled, single-blind, randomized, three-treatment, three-period, crossover study in participants with complete, removable well-fitting, well-made upper/lower dentures.

A randomised bite force study assessing two currently marketed denture adhesive products compared with no-adhesive control.

Unlike other oral care products, there are limited technologies in the denture adhesive category with the majority based on polymethyl vinyl ether/maleic anhydride (PVM/MA) polymer. Carbomer-based denture adhesives are less well studied, and there are few clinical studies directly comparing performance of denture adhesives based on different technologies. This single-centre, randomised, three-treatment, three-period, examiner-blind, crossover study compared a carbomer-based denture adhesive (Test adhesive) with a PVM/MA-based adhesive (Reference adhesive) and no adhesive using incisal bite force measurements (area over baseline over 12 hr; AOB) in participants with a well-made and at least moderately well-fitting complete maxillary denture.

Randomized Controlled Clinical Study to Determine the Oral and Dermal Tolerability of an Experimental Denture Wipe.

Purpose: To evaluate oral and dermal tolerance following use and user acceptability of an experimental denture-cleansing wipe. An exploratory objective was to develop a method to assess denture wipe effectiveness in removing debris from denture surfaces.

Materials And Methods: This was a single-center, randomized, controlled, parallel-group, examiner-blind study in participants with ≥1 full/partial denture.

Randomized exploratory study to measure ion release from calcium sodium phosphosilicate-containing dentifrice.

This exploratory study investigated salivary concentrations of silicon, calcium, sodium, and phosphorous over a 60-min time period following the use of a calcium sodium phosphosilicate (CSPS)-containing dentifrice. Participants brushed with a dentifrice containing 5% (w/w) or 0% (w/w) CSPS or swilled with a slurry containing 5% (w/w) CSPS/glycerol. Saliva samples were collected before, and 2, 5, 15, and 60 min after, product use and were analysed using inductively coupled plasma optical emission spectroscopy.

Effect of phytate and zinc ions on fluoride toothpaste efficacy using an in situ caries model.

Objectives: To compare and explore the dose-response of phytate-containing 1150 ppm fluoride toothpastes on model caries lesions and to determine the impact of zinc ions.

Methods: This was a single-centre, randomised, blinded (examiner/laboratory analyst), six-treatment, four-period crossover, in situ study in adults with a removable bilateral maxillary partial denture. Study treatments were toothpastes containing: 0.

Effects of a sodium fluoride- and phytate-containing dentifrice on remineralisation of enamel erosive lesions-an in situ randomised clinical study.

Objective: The objective of this work was to evaluate effects of a dentifrice containing sodium fluoride (1150 ppm F) and the organic polyphosphate phytate (0.85% w/w of the hexa-sodium salt) on in situ remineralisation of early enamel erosive lesions and resistance to subsequent demineralisation.

Materials And Methods: Subjects (n = 62) wore palatal appliances holding eight bovine enamel specimens with pre-formed erosive lesions.

A randomized clinical trial to evaluate the stain removal efficacy of a sodium phytate dentifrice formulation.

Objectives: Phytate is an organic, cyclic polyphosphate analogous to linear condensed polyphosphates used as stain removal agents. This study investigated stain removal efficacy of an experimental sodium phytate-containing dentifrice compared to a reference dentifrice.

Methods: An experimental, moderate abrasivity (relative dentine abrasivity [RDA] ∼130) antisensitivity fluoride dentifrice containing sodium phytate (0.

Potassium oxalate oral rinses for long-term relief from dentinal hypersensitivity: Three randomised controlled studies.

Objectives: To evaluate effectiveness of oral rinses containing dipotassium oxalate monohydrate (KOX) in relieving dentinal hypersensitivity (DH) after 8 w use adjunctive to brushing.

Methods: Three 8-week, randomised, controlled, double-blind, parallel-group, single-centre studies were conducted in adults with DH. In each study, participants were randomised to one of two experimental rinses or a placebo in a matrix design, such that each experimental rinse was evaluated in two studies.

Longitudinal correlation of 3D OCT to detect early stage erosion in bovine enamel.

Erosive tissue-loss in dental enamel is of significant clinical concern because the net loss of enamel is irreversible, however, initial erosion is reversible. Micro-hardness testing is a standard method for measuring initial erosion, but its invasive nature has led to the investigation of alternative measurement techniques. Optical coherence tomography (OCT) is an attractive alternative because of its ability to non-invasively image three-dimensional volumes.

In Vitro Effect of Porphyromonas gingivalis Methionine Gamma Lyase on Biofilm Composition and Oral Inflammatory Response.

Methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. The aim of this study was to compare an mgl knockout strain of P.

Structural and compositional changes in the salivary pellicle induced upon exposure to SDS and STP.

Sodium dodecyl sulphate (SDS) and sodium tripolyphosphate (STP) act to remove stained pellicle from dentition and loosen deposits on tooth surfaces that may become cariogenic over time. This study investigated how SDS and STP impact the salivary pellicle adsorbed onto hydroxyapatite and silica sensors using a dual polarisation interferometer and a quartz-crystal microbalance with dissipation. After the pellicle was exposed to SDS and STP the remaining pellicle, although weaker, due to the loss of material, became less dense but with a higher elastic component; suggesting that the viscous component of the pellicle was being removed.

In vitro growth characteristics and volatile sulfur compound production of Solobacterium moorei.

Solobacterium moorei has recently been implicated as a causative agent of halitosis. In vitro experiments to evaluate the role of S. moorei in halitosis have, however, been complicated by a paucity of information on the ideal conditions for culturing this organism.

Structural characterisation of parotid and whole mouth salivary pellicles adsorbed onto DPI and QCMD hydroxyapatite sensors.

In this study we investigated the differences in the properties of pellicles formed from stimulated parotid saliva (PS), which contains little or no mucin; and stimulated whole mouth saliva (WMS), which contains mainly two types of mucin: MUC5B and MUC7. By contacting WMS and PS with quartz-crystal microbalance with dissipation monitoring (QCM-D) and dual polarisation interferometer (DPI) hydroxyapatite (the main component of enamel) coated sensors, we observed the formation and structure of the respective salivary pellicles. As this was the first time that DPI hydroxyapatite sensors have been used to measure salivary pellicle adsorption; the techniques combined allowed us to measure the hydrated mass, dry mass, thickness and viscoelastic properties of the pellicle; but also to record the density of the PS and WMS formed pellicles.

The effect of an experimental anhydrous stannous fluoride dentifrice on the acid resistance of dentin smear layers.

Purpose: To compare the relative performance, in an in vitro assay, of a standard dentifrice (Colgate Cavity Protection) with an experimental anhydrous 0.454% w/w stannous fluoride dentifrice at protecting the dentin smear layer against dietary acids.

Methods: Artificial smear layers were created on bovine dentin, contacted with slurries of the dentifrices, then exposed to a dietary acid (Coca Cola) for periods up to 10 minutes.

In vitro studies investigating the dentin tubule-occlusion properties of an experimental anhydrous stannous fluoride dentifrice.

Purpose: To evaluate in vitro the ability for an experimental anhydrous 0.454% w/w stannous fluoride dentifrice to occlude dentin tubules.

Methods: Scanning electron microscopy (SEM) was used to assess tubule occlusion and the resistance of the occlusion to dietary acid in a 4-day model, where bovine dentin was treated with product twice daily with acid challenges administered on Days 3 and 4.

Effect of calcium ions on in vitro pellicle formation from parotid and whole saliva.

The salivary pellicle is a protein-rich, bacteria-free, self-assembling film that adsorbs to all surfaces within the oral cavity. The pellicle has numerous functions that are vital for maintaining oral health. Currently however, there are no commercially available artificial salivas that accurately mimic the complex film forming properties (i.

Chitosan microparticles for the controlled delivery of fluoride.

Objectives: To manufacture and characterise chitosan/fluoride microparticles prepared by spray drying and assess their utility as controlled release vehicles for fluoride.

Methods: Microparticles were manufactured from dispersions containing 1.0% and 2.