Clinical Outcome After Epidural Spinal Cord Stimulation in Patients With Severe Traumatic Brain Injury.

Introduction:  Epidural spinal cord stimulation is a minimally invasive procedure with a growing list of indications. It has a good safety profile and analgesic effect, reduces the severity of spasticity, and activates various brain regions. The purpose of this study is to evaluate the clinical outcome of epidural spinal cord stimulation in patients with spastic syndrome and chronic disorders of consciousness resulting from severe traumatic brain injury (sTBI).

Cytokines and neurotrophic factors in the severity assessment of children autism.

Due to the steady increase in the number of children with autism and the high heterogeneity of clinical groups, the diagnosis of these disorders and their severity is an urgent problem in modern medicine. In the course of the work, 126 children from 3 to 13 years old with typical neurodevelopment and with severe and mild autism spectrum disorders (ASD) were examined. Disease severity was determined according to the Childhood Autism Rating Scale (CARS).

Distribution of the HLA-DPB1 alleles in a Russian population living in the Chelyabinsk region (South Ural of Russia).

The purpose of this work was to establish the distribution of HLA-DPB1 alleles in Russians living in the Chelyabinsk region (Russia). DPB1 frequencies were determined in 100 unrelated Russian, living in the Chelyabinsk region. All subjects were healthy unrelated blood donors, between 18 and 55 years of age.

HLA gene and haplotype frequencies in a Nagaybaks population from the Chelyabinsk Region (Russian South Urals).

A total of 112 Nagaybaks, a Turkic ethnoconfessional group living mainly in the Nagaybak district of the Chelyabinsk Region of Russian South Urals, were genotyped for HLA-A, -B, -DRB1, -DQA1 and -DQB1 loci using PCR-SSP (low-resolution) and HLA-A29 (high-resolution). All loci were in Hardy-Weinberg equilibrium (all p values >0.1 thus showing no locus-level deviations.

Force measurements reveal how small binders perturb the dissociation mechanisms of DNA duplex sequences.

The force-driven separation of double-stranded DNA is crucial to the accomplishment of cellular processes like genome transactions. Ligands binding to short DNA sequences can have a local stabilizing or destabilizing effect and thus severely affect these processes. Although the design of ligands that bind to specific sequences is a field of intense research with promising biomedical applications, so far, their effect on the force-induced strand separation has remained elusive.

[DISTRIBUTION OF TWO-LOCUS HAPLOTYPES OF MICROBIAL COMPONENT SENSOR GENES TLR1 AND TLR6 IN MAJOR POPULATIONS OF SOUTH URALS].

Aim: Evaluate the fraction of various TLR1-TLR6 haplotypes in populations of Russians Bashkir and Nagaybak of Chelyabinsk Region.

Materials And Methods: Potential donors of stem cells from Chelyabinsk Region Station of Blood Transfusion registry were included into the study and split into 3 populations: Russians (81), Bashkir (78) and Nagaybak (84). Genotyping by 2 polymorphisms of TLR1 and TLR6 genes was carried out in all the 3 groups.

[Comparative characteristics of HLA system gene distribution in patients with lung tuberculosis of the Russian population of Chelyabinsk region].

Aim: Study of distribution of genes and HLA system haplotypes A, B, DRB1, DQA1, DQB1 in healthy individuals and patients with lung tuberculosis, members of the Russian population of Chelyabinsk region for isolation of risk markers for the development of various forms of tuberculosis.

Materials And Methods: The study group consisted of 86 patients with lung tuberculosis of the Tuberculosis Dispensary No. 3 of Chelyabinsk of Russian nationality.

[Soluble form of trigger receptor expressed on myeloid cells-1 as a marker of burn wound mixed infection].

Aim: Detection ofearly non-bacteriological markers ofburn wound mixed microbial infection.

Materials And Methods: The level of soluble form of trigger receptor expressed on myeloid cells-1 (sTREM-1) and cytokines in blood sera of 60 burn patients on days 3 - 6 and 10 - 17 after the burn was studied by solid phase enzyme immunoassay.

Results: At the early periods of burn disease the level ofsTREM- 1 in sera may be a non-bacteriological marker of burn wound mixed infection: at days 3 - 6 after the injury - higher than 298.

Ion mobility spectrometry reveals duplex DNA dissociation intermediates.

Electrospray ionization (ESI) soft desolvation is widely used to investigate fragile species such as nucleic acids. Tandem mass spectrometry (MS/MS) gives access to the gas phase energetics of the intermolecular interactions in the absence of solvent, by following the dissociation of mass-selected ions. Ion mobility mass spectrometry (IMS) provides indications on the tridimensional oligonucleotide structure by attributing a collision cross section (CCS) to the studied ion.

[The character of the morphological changes of the mucous membrane of the large intestine and the genetic polymorphism of IL-1RA, IL-1B, IL-4 TNFA in patient with irritable bowel syndrome].

Unlabelled: The aim of this study was to evaluate the presence or absence of a relationship between the variants of the course of IBS and their association with genetic polymorphisms of genes and intergenic interaction of cytokines.

Materials And Methods: The sample consisted of 81 patients, the diagnosis was verified according to the criteria of the Rome III, were isolated psychopathological, morphological complications, extra-intestinal symptoms. Polymorphism genotyping IL-1Ra, IL-b, IL-4, TNFa performed by PCR.

[Destabilization of homeostasis of organism of burn patients as a marker of sepsis development, its complications and outcome].

Aim: Detection in burn patients of markers of sepsis development, its complications and outcome.

Materials And Methods: Sera IL-6, TNF-alpha, IL-8, IL-1beta, IL-Ra, IL-18, INF-gamma, IL-10 and sTREM-1 cytokine levels of burn patients at days 3-6 and 10-17 after the burn were studied.

Results: Statistically significant cytokine parameters in blood sera that already at the early stages of the burn disease may be markers of the course severity and sepsis outcome were obtained.

HLA gene and haplotype frequencies in Russians, Bashkirs and Tatars, living in the Chelyabinsk Region (Russian South Urals).

We have characterized the HLA-A, -B, -DRB1, -DQA1 and -DQB1 profiles of three major ethnic groups living in Chelyabinsk Region of Russian South Urals, viz., Russians (n = 207), Bashkirs (n = 146) and Tatars (n = 135). First field level typing was performed by PCR using sequence-specific primers.

Temperature response of PNIPAM derivatives at planar surfaces: comparison between polyelectrolyte multilayers and adsorbed microgels.

Two strategies for the design of thermosensitive coatings based on poly-N-isopropyl acrylamide (PNIPAM) derivatives are presented: 1) polyelectrolyte multilayers containing a diblock copolymer with a large PNIPAM block and 2) adsorption of PNIPAM microgels. The multilayers show only a small but irreversible response to the increase of outer temperature due to the strong interdigitation between the charged part and the temperature-sensitive block, while the adsorbed microgels show a pronounced and reversible response. It will be shown that the microgel number density can be easily controlled at the substrate.

[Evaluation of the toxic properties of cyanobacteriae of the Shershnevo water storage basin in the Chelyabinsk Region].

The toxic properties of cyanobacteriae of the Shershnevo water storage basin have been studied. Over almost the whole vegetative period of 2005, the cyanobacteriae of the Shershnevo water storage basin may be characterized as high toxic (when intraperitoneally injected to male CBA mice, LD50 was less than 100 mg/kg). There were no interspecific differences between the noninbred animals in cyanotoxin susceptibility.

[Characteristics of urogenital and post-enterocolitis reactive arthritides].

The immune status and profile of HLA antigens of loci A and B were evaluated in 159 patients with reactive arthritis. Reactive arthritis was caused by urogenital chlamydial infection in 64.2% of cases and by Shigella, Salmonella and Yersinia enterocolitica infection in 18.

[Characteristics of lymphoid subpopulations of peripheral blood of patients with shigellosis].

This work presents some investigation results of lymphoid subpopulation functional activity: Tg+1, Tg-1, Theophylline-resistant and Theophylline-sensitive lymphocytes and O-1 isolated from the peripheral blood of patients with bacterial dysentery (BD) in local xenogenic GVHD. It has been established that Tg+1 and Tg-1 have stimulating effect on local xenogenic GVHD, but O-1 are inert though the quantity of Fcg+--receptor carrying lymphocytes among O-1 in BD and nonspecific ulcerative colitis is increased compared with donors. Theophylline-resistant and Theophylline-sensitive lymphocytes demonstrated inhibiting effect on GVHD formation which was evident in the latter case.

[Destabilization of the immune homeostasis in patients with nonspecific ulcerative colitis of varying degrees of severity].

As a result of evaluating immune homeostasis in patients suffering from nonspecific ulcerous colitis, the predictors of the patient's gravity have been established. They include: 1) phagocytic activity; 2) changes in bioenergetic processes of the cells; 3) redistribution of functional activity and its phenotypic expression among lymphocytes; 4) imbalance of humoral factors of immunity; 5) augmentation of anemia with decreased or increased iron content.

[The characteristics of the phenotype and lymphocyte function in patients with a mucosal lesion of the large intestine].

The results of studies of functional activity of lymphocyte subpopulations: theophylline-sensitive (thphs-1) and theophylline-resistant (thphr-1), separated from peripheral blood of healthy people and patients with nonspecific ulcerative colitis (NUC) are represented in this paper. The evaluation of the separated subpopulations was performed by local xenogeneic graft-VS-host reaction, study of distribution of nonspecific esterase in cells and determination of relative amount of Fc gamma-R positive lymphocytes among thphs-1 and thphr-1. It was established that lymphocytes of patients with NUC had an alternative form of action on development of xenogeneic graft-VS-host reaction in contrast with cells of donors, that is inhibited the reaction.

[Characteristics of lymphoid subpopulations isolated from healthy subjects and those suffering from nonspecific ulcerative colitis].

Results of the studies of functional activity of lymphocyte subpopulations T+G, T-G, "O" subpopulations isolated peripheral blood of healthy persons and those with nonspecific ulcerative colitis (NUC) have been presented in this paper. The functional activity of the cells was assessed by means of local xenogeneic graft-vs-host reaction performed on CBA mice and by determination of nonspecific esterase enzyme. It has been established that analogous lymphocyte subpopulations influence the graft-vs-host reaction development in different ways depending on the condition of cell donor state: health/pathology.

[The ratio of various lymphocyte populations in bacillary dysentery and nonspecific ulcerative colitis].

The comparative study of the content of different lymphocyte populations of the peripheral blood in pathological states accompanied by lesions of the mucous membrane of the large intestine has been made. In shigellosis patients the accumulation of lymphocytes having the signs of young post-thymic forms (theophylline-dependent populations) and functionally active forms (Ea-rosette-forming cells) occurs in the circulating blood. In unspecific ulcerous colitis only an increase in the number of immature lymphocytes (theophylline-dependent lymphocytes and autorosette-forming cells) is observed.

[Analysis of different lymphocyte subpopulations in patients with nonspecific ulcerative colitis].

A complex of methods based on a rosette forming reaction, was used to study the content of different lymphocytic populations in the peripheral blood of persons with nonspecific ulcerative colitis. Imbalance in the system of immunocompetent cells was shown to develop in this disease: a decrease in the amount of suppressors (T gamma and theophylline sensitive lymphocytes) and a increase in the amount of immature postthymic cells: cells forming rosettes with autologous erythrocytes, and theophylline dependent lymphocytes.

[Effect of ADT vaccine on children's nonspecific resistance to influenza and acute respiratory diseases when administered at different times before an outbreak of influenza].

The planned immunization of children aged 11 years with adsorbed DT vaccine in the years of influenza epidemics produced changes in unspecific resistance, occurring in phases: a decrease at the early postvaccinal period and an increase at the late postvaccinal period. When immunization was carried out, on the average, 10 days before the outbreak of influenza, the increased susceptibility phase coincided with the outbreak of influenza; as a reuslt, morbidity rate among the vaccinated children was 3 times higher than among the nonvaccinated children (P < 0.05).

[Change in the non-specific resistance of the body to influenza and acute respiratory diseases following immunization diphtheria-tetanus vaccine].

A single vaccination of animals with diphtheria-tetanus vaccine caused changes in non-specific resistance of the organism to influenza of phasic character: a reduction of resistance to influenza was observed at the early periods after the vaccination, with its subsequent restoration to the initial level and increase at the remote periods after the immunization. Immunization of children with diphtheria-tetanus vaccine also led to a short-term reduction non-specific resistance to influenza and acute respiratory diseases during the second decade after the vaccination, and its subsequent increase at periods from the 40th to the 60th day.