A new application of inorganic sorbent for biomolecules: IMAC practice of Fe-nano flowers for DNA separation.

Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribonucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers.

Co-evaluation of interaction parameters of genomic and plasmid DNA for a new chromatographic medium.

Preparation of new sorbents specific to DNA has a great significance in many biomedical fields. This study reports a new sorbent with high surface area and porosity to immobilize nucleic acids having both high molecular weight like genomic DNA (gDNA) for potential use in therapy of some immune system disease and low molecular weight like plasmid DNA (pDNA) for diagnosis, gene therapy and DNA vaccination. For this aim, silica-based pore-expanded SBA-15 nanoparticles with aminopropyl-trimethoxysilane (APTMS) for decoration of Fe ions (PE SBA-15-APTMS/Fe) were synthesized to get high surface area for high adsorption, and embedded into cryogel column for obtaining interconnected pores to avoid diffusion limitation of DNA samples because of their viscosity features.

Hybrid metal-organic nanoflowers and their application in biotechnology and medicine.

Nanoflowers - new nanostructures - have aroused the interest of scientists due to the topographic features of nanolayers, the special location of which allows a higher surface-to-volume ratio compared to classic spherical nanoparticles, which significantly increases the efficiency of surface reactions for nanoflowers. The main purpose of these types of nanomaterials is their use as enzyme stabilizers. To facilitate the functioning of enzymes under different conditions, organic-inorganic hybrid nanomaterials have been developed, the name of which indicates that all components of inorganic nanoparticles are associated with organic materials.

Comparison of therapeutic effects of sodium hyaluronate and corticosteroid injections on trapeziometacarpal joint osteoarthritis.

This was a randomized, open-label, evaluator-blinded clinical study including 40 women with stage II or III trapeziometacarpal joint osteoarthritis. The steroid group (n = 20) received one injection of 20 mg triamcinolone acetonide once and the hyaluronate group (n = 20) received three injections of 5 mg sodium hyaluronate at 1-week intervals. The pain level was assessed using a visual analog scale and grip and pinch strengths were measured using a hand grip dynomemeter and pinch gauge.