[A thin-layer chromatography method for determining the styrene metabolites mandelic and phenylglyoxylic acid in urine].

A thin-layer chromatographic method is described for the determination of mandelic and phenyglyoxillic acid on silicagel (Silufol UV 254) after extraction from urine of styrene exposed workers. The quantitative determination was performed after eluting the spots. Phenylglyoxilic acid was measured at 255 nm and mandelic acid by derivative spectroscopically estimation of the .

[Methods for enzymatic determination of triglycerides in liver homogenates].

An enzymatic method is described for the determination of triacylglycerols in liver homogenate. In contrast to usual methods, higher reliability and selectivity are achieved by omitting the extraction step.

[Renal excretion of total porphyrins and hippuric acid in rats].

The amounts of total porphyrins, hippuric acid and creatinine, excreted in urine by adult male Wistar rats, exhibited normal distributions for hippuric acid and creatinine, but a bimodal distribution for total porphyrins. This typical distribution of total porphyrins was still observed when creatinine was used as reference parameter. In biochemical and toxicological experiments in rats, the tested parameters should be therefore be investigated for homogeneity.

Combined action of hepatotoxic substances and increased environmental temperature on the liver of rats.

Male albino rats were orally treated in a single dose with 40 mg allyl alcohol/kg or 50 mg 4,4'-methylenedianilline/kg, or 100 mg 1,1,2,2-tetrachloroethane/kg, or 400 mg carbon tetrachloride/kg, or 800 mg chloroforme/kg, immediately after-wards exposed to increased temperature (35 degrees C, 50% RH, 4 h), and examined 20--22 hours later. Under these conditions, only carbon tetrachloride led to a more intensified changes manifesting themselves in changes of the serum enzymes LAP, ALAT, and GLDH, as well as in liver histology. The liver lesions, caused by the substances mentioned, reacted to an additional thermal strain in a comparable manner as the LD50-values determined under identical exposure conditions.

The effect of simultaneous oral application of ethanol and styrene. [1. Acute and subacute experiments on rats].

Within the lethal dose range, 8.70 mmol/ml ethanol (EtOH, LD50 = 226 mmol/kg) and 6.86 mmol/ml styrene (ST, LD50 = 77.

[Changes in the liver following a single administration of 11-H-eicosafluoroundecanoic acid in rats].

A single oral administration of 27 mg 11-H-eicosafluorundecanoic acid/kg of body weight (according to 1/20 of the oral LD50; 0.049 mM) resulted in a long lasting hepatomegaly in male rats. This was accompanied by a decrease of the triglyceride and total lipid levels in blood serum, a temporary increase of the total lipids in the liver, and an increase of the hexobarbital sleeping time, the latter being normalized before the liver/body weight ratio reached the control level.