HMGA2 expression distinguishes between different types of postpubertal testicular germ cell tumour.

The group of postpubertal testicular germ cell tumours encompasses lesions with highly diverse differentiation - seminomas, embryonal carcinomas, yolk sac tumours, teratomas and choriocarcinomas. Heterogeneous differentiation is often present within individual tumours and the correct identification of the components is of clinical relevance. HMGA2 re-expression has been reported in many tumours, including testicular germ cell tumours.

Detection of PAX8-PPARG fusion transcripts in archival thyroid carcinoma samples by conventional RT-PCR.

The t(2;3)(q13;p25) occurs in a subgroup of follicular-patterned thyroid tumors and leads to a fusion of the genes encoding for the thyroid-specific transcription factor paired box 8 (PAX8) and the peroxisome proliferator-activated receptor gamma (PPARγ). Although initially discovered in follicular carcinomas (FTC), the fusion transcripts were also detected in a small fraction of follicular adenomas and rarely in follicular variants of papillary carcinomas (FV-PTC). In most RT-PCR based studies, fresh or snap-frozen tissue samples were used.

Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.

Background: Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but the role of THADA in the thyroid is still elusive. The aim of this study was to quantify THADA gene expression in normal tissues and in thyroid hyper- and neoplasias, using real-time PCR.

Methods: For the analysis THADA and 18S rRNA gene expression assays were performed on 34 normal tissue samples, including thyroid, salivary gland, heart, endometrium, myometrium, lung, blood, and adipose tissue as well as on 85 thyroid hyper- and neoplasias, including three adenomas with a 2p21 translocation.

6p21 rearrangements in uterine leiomyomas targeting HMGA1.

To quantify the expression of HMGA1 mRNA in uterine leiomyomas, the expression of HMGA1 was analyzed in a series including tumors with aberrations of chromosome 6 (n = 7) and cytogenetically normal tumors (n = 8) as a control group by quantitative reverse transcriptase-polymerase chain reaction. The average expression level in the 6p21 group was found to be 5.6 times higher than that in the control group, and with one exception, all cases with 6p21 alteration revealed a high expression of HMGA1 mRNA than cytogenetically normal tumors.

Upregulation of HMGA2 in thyroid carcinomas: a novel molecular marker to distinguish between benign and malignant follicular neoplasias.

The identification of molecular markers allowing to differentiate between benign and malignant thyroid tumors remains a diagnostic challenge. Herein, we have used the expression of the high mobility group protein gene HMGA2 and its protein, respectively, as a possible marker detecting malignant growth of thyroid tumors. HMGA2 belongs to the high mobility group proteins, i.

[Immunological data in the clinical evaluation of Hodgkin's diseases].

87 adult patients with histologically confirmed diagnosis of Hodgkin's disease were examined to analyse the pattern of immune alterations with regard to the clinical stage and course of disease, and the response to treatment. Patients with advanced stages of the disease or with radio- or chemotherapeutically induced remission showed a decrease in total lymphocyte counts and a diminished count of T-lymphocytes. The lymphocyte transformation and the reactions in skin tests with DNCB and microbial antigens were the more disturbed the more the disease was advanced.

Effect of cyclophosphamide on the immune response to sheep erythrocytes in the mouse. II. A study of enzyme activities in the draining lymph node.

Young adult CFW mice, injected with sheep erythrocytes, were treated with cyclophosphamide and sacrificed at different times. The activity of acid phosphatase and for non-specific esterase was measured by semi-quantitative methods in smears prepared from cell suspension of draining lymph node. In cyclophosphamide-treated mice was noted a significant decrease in acid phosphatase activity in lymphoid cells, expressed both as average activity in single lymphoid cell and a decrease in proportion of cells exhibiting enzyme activity.