Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues.

Meloidogyne enterolobii, a guava root-knot nematode, is a highly virulent pest in tropical and subtropical regions causing galls or knots in roots of diverse plant species posing a serious threat to agriculture. Managing this nematode is challenging due to limitations in conventional identification based on isolation and microscopic classification requiring expertise and time. A colorimetric and fluorescent LAMP assay using simplified extraction method targeting rDNA-ITS region was developed to detect M.

Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway.

Activation of induced plant resistance to control pests and diseases is regaining attention in the current climate where chemical pesticides are being progressively banned. Formulations of chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), COS-OGA, have previously been described to induce resistance against fungal diseases in different crop plants. Here, we investigated their potential and mode-of-action as preventive measures to control root-knot nematode Meloidogyne graminicola infection in rice.

Activity of Vetiver Extracts and Essential Oil against .

Vetiver, a nonhost grass for certain nematodes, was studied for the production of compounds active against the southern root-knot nematode, Meloidogyne incognita . In laboratory assays studying the effects on second-stage juvenile (J2) activity and viability, crude vetiver root and shoot extracts were nematotoxic, resulting in 40% to 70% J2 mortality, and were also repellent to J2. Vetiver oil did not exhibit activity against J2 in these assays.