[Lymphocytes with receptors to streptococcal polysaccharide group A in the thymus of rheumatism patients. Change in the response of normal thymocytes to adenosine, theophylline and decantate].

With the help of immunomodulators (adenosine, theophylline, levamisole) and decantat of 3-hour culture of normal thymocytes, the features of thymocytes with the receptor for polysaccharide of streptococcus group A(A-PSC) (R = PSC+cells) in patients with rheumatism. It has been established that in patients' thymus the quantity of lymphocytes, able to express R-PSC (predecessors of R = PSC + cells) decreased under the influence of theophylline and adenosine. The predecessors of R = PSC+thymocytes in the majority of patients with rheumatism are areactive to decantat action.

[Disordered cell-receptor metabolism (R-proteins) and the immunity indices in patients with lymphoproliferative diseases of the skin and Kaposi's sarcoma].

Elevated blood serum concentrations of R protein were revealed in the patients with malignant lymphomas of the skin and with Kaposi's sarcoma. In both conditions the level of R protein was elevated as against the norm and related to some cellular and humoral immunity parameters. Measurement of R protein concentrations and of the levels of some cellular and humoral immunity parameters in malignant lymphomas of the skin and Kaposi's sarcoma evidence a prognostic value of R proteins.

[The pathogenesis of dermatoses due to contact with mineral fertilizers].

The studies have revealed unfavourable effect of mineral fertilizers on the peripheral blood cells. A complex of tests was used to diagnose dermatoses induced by exposure to mineral fertilizers.

[The determination of receptor proteins in patients with mycosis fungoides].

Measurements of the blood serum R-protein levels in patients with mycosis fungoides have revealed their elevation by 2-10 times as against healthy donors. Referon therapy combined with prospidine and indomethacin resulted in improvement of the clinical course of the process and was accompanied by a reduction of R-protein level. These data suggest that measurements of R-protein levels may help monitor the treatment efficacy.

[Receptors for polysaccharides of group A streptococci on human thymic lymphocytes. Stimulation of their expression by action of adenosine, theophylline and supernatant of thymocytes].

It was established by indirect immunofluorescence that thymic lymphocytes bear receptors for polysaccharide of group A streptococci (Rps). The ability of thymic lymphocytes to express Rps depends on the cAMP concentration in the cell, because the treatment of thymocytes with adenosine and theophylline increases the number of cells with Rps (Tps cells). Supernatant of thymic lymphocytes is also capable of stimulating expression of Rps.

[The role of non-type-specific protein antigens of the cell wall in Streptococcus group A in developing delayed hypersensitivity].

The role of the type-nonspecific (TNS) cell-wall antigens of group A streptococci has been determined. The study has been made on guinea pigs sensitized with whole microbial cells or HCl extracts containing TNS antigens. To determine delayed hypersensitivity, the in vitro cytotoxic test on adhering lymph-node cells in the autologous system has been used.

[Receptors for the secretory component on human thymic lymphocytes. Stimulation of their expression as affected by adenosine, theophylline and a thymic lymphocyte supernatant].

It has been established by indirect immunofluorescence that thymic lymphocytes bear receptors for secretory component (Rsc). The bound secretory component, i. e.

[Collagen type IV of the basal membrane of the epithelium of the skin in certain forms of pathology].

Using immunofluorescence, attenuation or complete disappearance of type IV collagen antiserum reaction were shown in the epidermal basal membrane zone of SLE patients. Some positive material was revealed in the intercellular spaces of the epidermis. There is an inverse correlation between the amount of immune complexes in the dermo-epidermal region and the intensity of anticollagen serum reaction in the basal membrane zone.

[Stimulation of expression of IgM and IgG receptors on human thymus lymphocytes after treatment with lactoferrin in vitro].

It was established by immunofluorescence that lactoferrin, one of the heteroorganic thymus antigens, can stimulate the expression of Fc mu and Fcj receptors on thymus lymphocytes. The stimulating effect of lactoferrin on T mu cells is more pronounced with the level of these cells in the thymus being low. Its effect on Tj cells seems independent of their level in the thymus and may be related to their precursor differentiation.

[Use of germ-free mice in experimental transplantation].

A comparative study of skin allografts was performed in germ-free A/Ola mice kept in boxes and in A/Ola and BALB mice raised in ordinary conditions. Skin graft (of C57B1/6 mice) in A/Ola and BALB mice raised in ordinary conditions was shown to reject 16-21 and 12-18 days after the transplantation, respectively without cyclophosphamide (CP) use. CP application in BALB mice, grown in ordinary conditions, prolonged the lifespan of grafts to 12-29 days.

[Comparative immunochemical and immunocytochemical study of 3 soluble granulocytic proteins in blood cells and serum].

Using immunofluorescence, it has been shown that leukocyte thermostable alpha-glycoprotein (LTAG) and leukocyte beta-globulin (LBG) are, like lactoferrin, components of polynuclear leukocytes. These proteins were found in the perinuclear zone, cytoplasm membrane and extracellularly. Serum LTAG concentration increases in immune inflammatory diseases.

[Cytotoxic effect of lymphocytes on autologous blood monocytes in the presence of streptococcal group A antigens in patients with primary erysipelas].

The present work deals with a modification of the cytotoxic test for the determination of the cytotoxic activity of lymphocytes in infectious diseases. This modification is based on the use of the suspension of mononuclear blood cells, simultaneously containing effector cells (sensitized lymphocytes) and target cells (autologous monocytes). The cytotoxic effect on monocytes is observed after the preliminary incubation of nonadhering cells (lymphocytes) with the antigen of microorganisms causing the infectious process.

[Reaction of loose connective tissue fibroblasts after poly-4-vinylpyridine administration].

Mice were injected with poly-4-vinylpyridine at a dose known to be effective for immunogenesis stimulation. Within 24 hours after the injection fibroblasts reacted by dendrit production, cytoplasm vacuolization and clasmocytosis. 2-3 days after the polymer injection the fibroblast cytoplasm was restored.

[Localization of basal cell antigen in the epithelial tissues of animals and humans detected by means of natural antibodies].

By means of the immunofluorescent method using rabbit serum that contains natural antibodies against the basal cell antigen of epidermis, the distribution of the antigen has been demonstrated in cells of the basal layer of all types of the stratified epithelium. The reaction is also noted in cytoplasm of the epithelial cells in the thymus and the tracheal mucous membrane. This demonstrates their histogenic affinity to stratified epithelii.

[Effect of hemosorption on the dynamics of immunopathologic changes in the skin of patients with diseases accompanying the immune complex syndrome].

In the majority of diseases with the immune complexes (IC) syndrome, elimination of IC from the body is markedly disturbed. The authors carried out a study with the aid of the direct immunofluorsecence technique. The use of hemosorption in the treatment of patients with grave patterns of systemic lupus erythematosus, rheumatoid arthritis, psoriasis, systemic vasculitis and similar illnesses in conjunction with conventional therapy (corticosteroids, cytostatics) leads to sequestration of the depositions of immune complexes from the skin.

[Immunological changes in the thymus epithelial tissue of mice during accidental involution].

Using sera containing antibodies to antigens of epithelial reticular cells it is possible to detect by immunofluorescence early stages of organ accidental involution and to trace parenchyma demasking that results from lymphocyte migration upon injection of prednisolone, azathioprin or their combinations. Prior to the drug administration only certain short thin processes of epithelial cells masked by a great number of lymphoid elements are detectable in the cortical zone of the thymus. Injection of 10-40 mg/kg prednisolone, 100-200 mg/kg azathioprin or their combinations causes stable different-degree demasking of the epithelial reticulum seen during immunofluorescence as a network of fluorescent processes.

[Receptors for lactoferrin on human thymic lymphocytes. The stimulation of expression as affected by adenosine, theophylline and a supernatant of thymic lymphocytes].

It was established by indirect immunofluorescence with the use of antibodies to human lactoferrin that thymic lymphocytes bear lactoferrin receptors in the point structures on the cell surface. The ability of thymic lymphocytes to express lactoferrin receptors depends on the cAMP concentration in the cell, inasmuch the treatment of lymphocytes with adenosine and theophylline increases the number of cells bearing lactoferrin receptors. Supernatant of thymic lymphocytes is also capable of stimulating expression of lactoferrin receptors.

[Demonstration of secretory component in membrane structures of the human thymus].

A large amount of secretory component (Sc) was demonstrated in human thymus by the immunofluorescence technique. The component was found to be contained by the membranes permeating the parenhuyma and surrounding numerous tubular formations in the cortical layer of the organ under discussion. It is suggested that like other heteroorgan antigens, secretory component is involved in the formation of natural immunological tolerance, informing organ lymphocytes of the structures of the host own antigens.

[Fibronectin in the structures of total preparations of loose connective tissue].

A comparative study of fibronectin localization in the cells and paraplastic structures of total film loose connective tissue preparations obtained from different animal species (mice, rats, guinea-pigs, golden hamsters) made it possible to reveal some regularities. In mice and rats, protein was found in fibroblast cytoplasm (or on its surface) in the form of dots and dotted lines. In golden hamsters, it was found in the form of larger round loci, whereas in guinea-pigs, fibronectin was diffused.