Publications by authors named "Buket S Bozkurt"
SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs).
J Periodontal Res
August 2021
Background/objectives: In this in vitro study, the effects of Stromal cell-derived factor-1 (SDF-1) was evaluated on the periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs) functions.
Material And Methods: Real-time cell analyzer-single plate (RTCA-SP) was employed for proliferation, and RTCA-dual purpose (DP) was utilized for pdl-MSCs migration potential treated with different SDF-1 concentrations (0, 0.1, 1, 10, 100, 200, and 400 ng/ml).
Real-time cell analysis of the cytotoxicity of orthodontic brackets on gingival fibroblasts.
J Appl Biomater Funct Mater
December 2014
Purpose: The aim of this study was to evaluate the cytotoxic effects of 6 different orthodontic bracket types on human gingival fibroblasts (HGFs) using the xCELLigence system.
Methods: The orthodontic brackets used in this study were gold-plated steel (Apollo Gold), titanium (Rematitan), stainless steel (Equilibrium 2), lucid ice (Inspire ICE), metal-reinforced ceramic (Clarity) and composite (OrthoFlex). Gingival fibroblasts were isolated from human gingival connective tissue of systemically healthy individuals.
Article Synopsis
- The study investigates how periodontal ligament (PDL) cells behave in response to different types of titanium surfaces, focusing on cell growth, shape, and mineralization of tissue.
- PDL cells were grown on smooth and various sandblasted titanium surfaces, with key experiments measuring cell proliferation and specific protein expressions using advanced microscopy and gene analysis methods.
- Results showed that while most surfaces had similar cell growth, the sodium titanate (NaTi) surface significantly influenced mineralization and increased expression of certain genes related to bone formation, suggesting it could be a better option for implants compared to other surfaces.
Introduction: The aim of this study was to evaluate the cytotoxic effects of orthodontic mini-implants on gingival fibroblasts and osteoblasts.
Methods: The orthodontic mini-implants used in this study were Orthodontic Mini Implant (Leone, Florence, Italy), MTN (MTN, Istanbul, Turkey), AbsoAnchor (Dentos, Daegu, South Korea), IMTEC Ortho (3M Unitek, IMTEC, Ardmore, Okla), VectorTAS (Ormco, Glendora, Calif). The materials were incubated in Dulbecco's modified eagle's culture medium for 72 hours according to ISO 10993-5 standards (surface area-to-volume ratio of the specimen to cell-culture medium, 3 cm(2)/mL).
Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts.
Am J Orthod Dentofacial Orthop
November 2011
Introduction: The aim of this study was to evaluate the cytotoxicity of 3 orthodontic acrylic materials and 2 manipulation methods.
Methods: The orthodontic acrylic materials Orthocryl EQ (Dentaurum, Ispringen, Germany), Orthoplast (Vertex Dental, Zeist, The Netherlands), and O-80 (Imicryl, Konya, Turkey) were prepared with 2 polymerization methods (doughing and spray on). Totally, 60 cylinders (5 × 2 mm), fabricated by using a different acrylic and method, were divided into 6 groups.
Boron regulates mineralized tissue-associated proteins in osteoblasts (MC3T3-E1).
J Trace Elem Med Biol
October 2010
The aim of this study was to determine the effects of boron (B) on the cell-survival, proliferation, mineralization and mRNA expression of mineralized tissue-associated proteins. Additionally, determination of the effects of B on the BMP-4, -6 and -7 protein levels of pre-osteoblastic cells (MC3T3-E1) was also intended. The effects of B (pH 7.
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