Sensitive amperometric biosensor for phenolic compounds based on graphene-silk peptide/tyrosinase composite nanointerface.

New graphene-silk peptide (Gr-SP) nanosheets were prepared and successfully fabricated with tyrosinase (Tyr) as a novel biosensor for the determination of phenolic compounds. The Gr-SP nanosheets were fully characterized with transmission electron microscopy, x-ray diffraction, x-ray photoelectron spectroscopy, UV/Vis and FTIR spectra. The developed biosensors were also characterized with scanning electronic microscopy and electrochemical impedance spectroscopy.

Effect of selenium on the interaction between daunorubicin and cardiac myosin.

The interactions between selenium (sodium selenite), anthracycline antibiotics daunorubicin (DNR), and major contractile protein cardiac myosin (CM) were investigated. The results showed that the binding force between selenium and CM was 100 times stronger than that of DNR and CM. There was no marked influence on fluorescence intensity of DNR-CM at selenium concentrations of up to 20 μM.

Electrochemical fabrication of molecularly imprinted porous silicate film electrode for fast and selective response of methyl parathion.

A methyl parathion-templated molecularly imprinted porous silicate thin film was electrodeposited onto a glassy carbon electrode using tetraethylorthosilicate sol as the silicon precursor and vinyltriethoxysilane as the functional monomer. The surface morphology and crystallinity of the imprinted film were characterized by scanning electron microscope and X-ray diffraction. The binding performance of the film with methyl parathion was examined with voltammetric techniques.

Isolation and fluorescence spectrum of cardiac myosin from pig heart.

Cardiac myosin is the most abundant and bioactive protein presented in heart tissue. In this work, a simple method was described for preparation of high purity cardiac myosin for further research. With the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.

Polymer modified biomass of baker's yeast for enhancement adsorption of methylene blue, rhodamine B and basic magenta.

In this study, poly (methacrylic acid) modified biomass was prepared to improve the adsorption capacities for three dyes: methylene blue (MB), rhodamine B (RB) and basic magenta (BM). FTIR and potentiometric titration demonstrated that a large number of carboxyl groups were introduced on the biomass surface, and the concentration of the functional group was calculated to be 1.4 mmol g(-1) by using the first and second derivative method.

Poly(amic acid)-modified biomass of baker's yeast for enhancement adsorption of methylene blue and basic magenta.

In this study, poly(amic acid)-modified biomass was prepared to improve the adsorption capacities for two cationic dyes, methylene blue and basic magenta. X-ray photoelectron spectroscopy and potentiometric titration demonstrated that a large number of imide, amine, and carboxyl groups were introduced on the biomass surface, and the concentrations of these functional groups were calculated to be 0.27, 1.

Effect of zinc and copper on the interaction of daunorubicin with cardiac myosin.

Daunorubicin (DNR) is an anthracyline antibiotic which induces a well-described but incompletely understood cardiac toxicity. In this study, a direct action of DNR on the major contractile protein, cardiac myosin (CM), was described utilizing the fluorescence spectroscopy. The quenching mechanism was suggested to be static quenching according to the fluorescence measurement.

Enhanced and selective adsorption of Pb2+ and Cu2+ by EDTAD-modified biomass of baker's yeast.

Enhanced and selective removal of Pb2+ and Cu2+ in the presence of high concentration of K+, Na+, Ca2+ and Mg2+ were achieved by adsorption on biomass of baker's yeast modified with ethylenediaminetetraacetic dianhydride (EDTAD). The modified biomass was found to have high adsorption capacities and fast rates for Pb2+ and Cu2+, and it also displayed consistently high levels of metal uptake over the pH range from 2.7 to 6.

Cystine-modified biomass for Cd(II) and Pb(II) biosorption.

The surface of dried biomass of baker's yeast was modified by crosslinking cystine with glutaraldehyde. X-ray photoelectron spectroscopy and microscope were used to characterize the modified biomass. The adsorption capacity of the modified biomass for Cd(2+) and Pb(2+) showed an increase compared with the pristine biomass due to the presence of cystine on the biomass surface.