Publications by authors named "Bugeon S"
Article Synopsis
- Transcriptomics has identified a variety of molecular subtypes among cortical inhibitory neurons, but their activity patterns in the living brain are still unclear.
- Research using in vivo imaging and transcriptomic analysis of mouse primary visual cortex (V1) found that the activity of inhibitory neuron subtypes correlates with brain states, primarily organized by a factor related to transcriptomic variation.
- Different subclasses of inhibitory neurons showed distinct responses to visual stimuli; those firing more during rest displayed specific physical and functional characteristics, illustrating a foundational principle governing how these diverse subtypes influence cortical processing based on brain state.
Article Synopsis
- Researchers studied how a gene called NEUROD2 affects brain development in mice and found that when this gene is missing, brain cells don't develop properly.
- Mice without NEUROD2 showed issues like being overly active, having trouble interacting with others, and even having seizures.
- The study also linked problems in these mice to human cases of developmental disorders, like autism, suggesting that NEUROD2 is important for normal brain function and development.
Neuronal activity has been identified as a key regulator of neuronal network development, but the impact of activity on migration and terminal positioning of interneuron subtypes is poorly understood. The absence of early subpopulation markers and the presence of intermingled migratory and postmigratory neurons make the developing cerebral cortex a difficult model to answer these questions. Postnatal neurogenesis in the subventricular zone (SVZ) offers a more accessible and compartmentalized model.
View Article and Find Full Text PDFAdult neurogenesis in the olfactory bulb (OB) is considered as a competition in which neurons scramble during a critical selection period for integration and survival. Moreover, newborn neurons are thought to replace pre-existing ones that die. Despite indirect evidence supporting this model, systematic in vivo observations are still scarce.
View Article and Find Full Text PDFbrain electroporation of DNA expression vectors is a widely used method for lineage and gene function studies in the developing and postnatal brain. However, transfection efficiency of DNA is limited and adult brain tissue is refractory to electroporation. Here, we present a systematic study of mRNA as a vector for acute genetic manipulation in the developing and adult brain.
View Article and Find Full Text PDFThe developmental mechanisms regulating the number of adult neural stem cells (aNSCs) are largely unknown. Here we show that the cleavage plane orientation in murine embryonic radial glia cells (RGCs) regulates the number of aNSCs in the lateral ganglionic eminence (LGE). Randomizing spindle orientation in RGCs by overexpression of Insc or a dominant-negative form of Lgn (dnLgn) reduces the frequency of self-renewing asymmetric divisions while favoring symmetric divisions generating two SNPs.
View Article and Find Full Text PDFBackground: The interpretation of 99mTc diethylenetriamine pentaacetate (99mTc DTPA) aerosol clearance is based on the hypothesis that the 99mTc-DTPA complex is not altered by the nebulization process.
Objectives: To characterize (1) the radiochemical purity (RCP) of 99mTc-DTPA and the stability of labeling after jet nebulization, and (2) the particle size distribution of the aerosol.
Methods: RCP and stability--the aerosol was driven by oxygen, captured on filters which were eluted and RCP was checked by thin layer chromatography.