Publications by authors named "Buddy E Early"
Article Synopsis
- A nine-locus STR multiplex system called GenePrint PowerPlex 2.1, developed by Promega, was tested alongside the Penta D locus to improve human identification accuracy.
- The system incorporates highly variable DNA loci and can work with the previously established PowerPlex 1.1 system, sharing some loci for quality control.
- Validation studies across seven laboratories confirmed the PowerPlex 2.1/Penta D system's efficiency, reproducibility, and reliability for forensic DNA testing, aligning with FBI quality assurance standards.
J Forensic Sci
March 2002
Article Synopsis
- - The NCSBI found discrepancies in DNA analysis of the D16S539 locus compared to other loci when outsourcing to TBTG, highlighting issues like allele drop out and imbalances in sister alleles.
- - A review of the differing lab techniques revealed that variations in DNA extraction and amplification methods could be causing the discrepancies, but the exact cause was unclear at first.
- - Further experiments identified a point mutation in the D16S539 primer-binding site that affects African-American populations, prompting Promega to create a new degenerate primer for more accurate detection of alleles in future tests.
