Development of a specific radioimmunoassay for the detection of clenbuterol residues in treated cattle.

A radioimmunoassay for clenbuterol detection in cattle has been validated and used to monitor treated cattle. The tracer used was 4-amino-3,5-dichloro-alpha(tert-butylamino-methyl) benzyl alcohol (benzyl-3H)(clenbuterol) prepared by catalytic tritiation with tritium gas of 4-amino-3,5-dibromo-alpha-(tert-butylamino)-acetophenone, followed by chlorination at positions 3 and 5 in the aromatic ring. The rabbit antiserum was raised against a diazotized clenbuterol/human serum albumin conjugate.

Reproductive performance of dibromochloropropane-treated female rats.

Dibromochloropropane (DBCP) is an effective nematocide which has been shown to suppress spermatogenesis and cause infertility in both men and male rats. There are no similar reports concerning the effects of DBCP on female reproduction. The purpose of the present study was to attempt to interfere with the various phases of oogenesis.

Gonadotoxicity and kinetics of dibromochloropropane in male rats.

Adult male rats were injected with [3H]dibromochloropropane dissolved in dimethylsulfoxide containing about 10 X 10(6) dpm. Blood from the tail and 24-h urine samples were collected up to 2 days post-injection. Tissue samples were further taken from the kidneys, liver, spleen, adrenals, epididymis, seminal vesicles and testes 7 h and 7 days post-injection.

Dopamine D2 receptors selectively labeled by a benzamide neuroleptic: [3H]-YM-09151-2.

In order to label dopamine D2 receptors selectively we tritiated the potent benzamide neuroleptic, YM-09151-2 (26.7 Ci/mmol). The binding of [3H]-YM-09151-2 to canine striatal membranes was saturable and specific with a KD of 57 pmol/l and Bmax of 36 pmol/g tissue as determined by Scatchard analysis.

Structure assignment by retention index in gas-liquid radiochromatography of substituted cyclohexenes.

Substituents and functional groups cause increase in retention indexes of six-membered ring compounds on apolar and polar columns. These retention index increments (delta I) are characteristic and structure-dependent showing a structure-retention index relationship. The delta I values obtained for methyl and halogen atom substitution, cyclohexenes, and cyclohexanes are useful for predictive purpose in tritium labeling studies for tentative structure assignment of radioactive intermediates not amenable to analysis by conventional techniques.