Publications by authors named "Bryan Puentes"
Article Synopsis
- There is a need for better animal models to study meibomian gland dysfunction (MGD) and evaporative dry eye disease (EDED), leading researchers to evaluate Awat2 knockout (KO) mice for this purpose.
- The study involved various tests on both wildtype and Awat2 KO mice, including ocular examinations, lipid analysis, and gene expression assessments.
- Results showed that Awat2 KO mice exhibited characteristics similar to human MGD and EDED, such as corneal issues and impaired meibum secretion, making them suitable for testing new treatments.
Purpose: The aim of this study was to develop and validate a method to generate phenol red thread tests (PRTT) due to the lack of availability of commercial PRTT.
Methods: White cotton thread was dyed with phenol red (pH indicator) for 48 h, dried, cut, and sterilized. To validate its wicking ability, the thread was inserted into solutions of varying pH, flanking the pH of healthy tears, for different time intervals.