Ecotoxicol Environ Saf
October 2023
Environmental exposure to metals can induce cytotoxic and genotoxic effects in cells and affect the health of the exposed population. To investigate the effects of aluminum (Al) and manganese (Mn), we evaluated their cytogenotoxicity using peripheral blood mononuclear cells (PBMCs) exposed to these metals at previously quantified concentrations in groundwater intended for human consumption. The cell viability, membrane integrity, nuclear division index (NDI), oxidative stress, cell death, cell cycle, and DNA damage were analyzed in PBMCs exposed to Al (0.
View Article and Find Full Text PDFThe Aquidauana River is an important ecological corridor in the Pantanal biome. However, the growth of agricultural and urban areas along its banks has contributed to the deterioration of its water quality, consequently putting the aquatic biota at risk. Our objectives were to evaluate: 1) the composition of the landscape around six sampling sites located in the Aquidauana River middle section; and 2) the quality of its water by determining limnological parameters, concentrations of contaminants of emerging concern (CECs), and the risks to native aquatic biota.
View Article and Find Full Text PDFThe Araguari River, one of the most important waterways in the Brazilian state of Amapá, is ecologically relevant and essential for the conservation of Amazonian fish biodiversity. Our previous studies demonstrated contamination of water and fish by metals. In particular, water samples showed genotoxic damage in Danio rerio.
View Article and Find Full Text PDFThe Aquidauana River is one of the most important rivers in the Pantanal region, Brazil. However, its waters have been contaminated by nearby anthropogenic activities, threatening native fish species. In this study, our objectives were: 1) to determine the concentrations of Al, As, Cd, Cu, Fe, Mn, Ni, Pb, and Zn in water and sediment samples from the Aquidauana River and to assess the risks posed to aquatic biota; 2) to quantify the concentration of these elements in muscle and liver tissue samples from four native fish species; 3) to evaluate the potential bioaccumulation of inorganic elements in the muscles and liver; and 4) to investigate genotoxicity biomarkers and their association with the inorganic element concentrations present in the muscle tissue.
View Article and Find Full Text PDFRecently, high concentrations of metals have been found in the waters from sampling sites located in the Araguari River lower section. These metals can be bioaccumulated by fish, but also biomagnified, representing a potential risk through human consumption. Here, we aimed to (1) determine the metal concentrations in muscle samples from fish; (2) evaluate bioaccumulation of metals in muscle tissue of fish species with different feeding behaviors; and (3) investigate the risks of single metals, as well as mixture of metals, to human fish consumption.
View Article and Find Full Text PDFSchinus terebinthifolia is a species native to different ecoregions in the Brazilian Atlantic Forest. The plant is listed on the National Relation of Medicinal Plants and recommended as phytomedicine, however while extractive exploitation prevails as the main route of raw material a significant variation of compounds will be detected. To assure the expansion of productive chain it is important to start by studying population diversity and chemical variations.
View Article and Find Full Text PDFChemosphere
December 2021
The Araguari River is one of the most important water body in the Brazilian state of Amapá. However, the quality of its waters has been deteriorating and, recently, high concentrations of metals were found in water samples collected from its lower section. Overall, we aimed to evaluate land use and cover around three sampling sites located in the Araguari River lower section and its contribution to water pollution by metals; correlate land use pattern with detected metal concentrations; assess acute toxicity using the Daphnia similis test and genotoxicity using the Danio rerio bioassay of water samples from the three sampling sites; and investigate the risks arising from metals present in waters to aquatic biota.
View Article and Find Full Text PDFThe objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.
View Article and Find Full Text PDFRecent molecular tools and technologies have marked the discovery of the origin and domestication processes of a wide variety of species, using either genomic or mitochondrial molecular markers to provide input for selection programs, as well as the management and conservation of animal breeds. This study aimed to analyze genes of mitochondrial DNA of the following sheep (Ovis aries) breeds: Pantaneira, Bergamácia, Dorper, White Dorper, Ile de France and Hampshire Down, to obtain their population genetic parameters and investigate the origin of these sheep populations reared in the State of Mato Grosso do Sul. The analysis of mitochondrial DNA allowed to infer their phylogenetic relationships and revealed significant differences among them when compared with each other and with sequences obtained from GenBank.
View Article and Find Full Text PDFAedes aegypti is the main mosquito vector of dengue, zika, chikungunya, and yellow fever diseases. The low effectiveness of vector control options is mainly related to the increased insect's resistance and to the toxicity of products used for non-target organisms. The development of new environmentally friendly and safer products is imperative.
View Article and Find Full Text PDFThis study assessed the quality of vegetation cover in the Parque Estadual das Várzeas do Rio Ivinhema (PEVRI, Upper Paraná River basin, MS, Brazil), the concentration of metals (Cd, Pb, Cr, Cu, Fe, Zn and Ni) in water and muscle and hepatic tissues of five fish species collected in the PEVRI - Hemiodus orthonops, Leporinus friderici, Prochilodus lineatus, Pterodoras granulosus and Pimelodus maculatus - in addition to non-carcinogenic risk assessment and genotoxicity potential in these species. Regarding vegetation index, we found that only 26.25% of the PEVRI area is occupied by denser vegetation.
View Article and Find Full Text PDFPremise: A novel set of nuclear microsatellite markers was developed and characterized for (Myrtaceae) and tested for cross-amplification in the related species .
Methods And Results: Forty-one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.
In the present study, we evaluated the effects of biological factors, lifestyle factors, and environmental conditions on the induction of DNA damage in exfoliated cells of the oral mucosa. Age, sex, medication use, and environmental conditions were analyzed in individuals residing in the cities of Caarapó and Itaporã. The individuals were assessed by a questionnaire, and oral mucosa cells were collected and subjected to mutagenicity analysis.
View Article and Find Full Text PDFGroundwaters are normally consumed without previous treatment and therefore the monitoring of contaminants in order to guarantee its safety is necessary. Thus, we aimed to evaluate the groundwater contamination by metals and emerging contaminants, seeking to understand the relationship between their presence in the groundwater and the use and land cover profile of Itaporã and Caarapó. In addition, the contaminant concentrations observed were compared with maximum permitted values (MPV) and/or with calculated water quality criteria (WQC) for human consumption to investigate possible human health risks due to the groundwater intake.
View Article and Find Full Text PDFThe objective of this study was to evaluate toxicogenetic potential of surface water samples from rivers of center-west Brazil and analyze the influence of land use and cover and physicochemical parameters in genetic damage. Samples were collected during winter (June) and summer (November) at sampling sites from Dourados and Brilhante Rivers (Mato Grosso do Sul/Brazil). The toxicogenetic variables, including chromosomal alterations, micronuclei, and mitotic index, were analyzed in meristematic cells of Allium cepa; and micronuclei, nuclear abnormalities, and DNA strand breaks (arbitrary units, AU) were analyzed in erythrocytes of Astyanax lacustris.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
March 2019
The use of chemical substances for the management of fish farming activities may compromise the quality of the tank water itself and of water bodies that receive the effluents. As studies that assess the environmental effect caused by pisciculture are scarce, the present study aimed at evaluating the water quality in two fish farms in the region of Grande Dourados, Brazil, from the site of water collection to the site of water disposal. The tools used for this purpose were the analysis of land use and cover and the determination of physical, chemical, and biological parameters of water samples.
View Article and Find Full Text PDFThe streams and rivers of the Upper Paraná River Basin have been seriously affected by impacts of high population density around the basin area. Fishes are widely used as models to assess the health of aquatic ecosystems, being considered as bioindicators of environmental pollution. In this context, our objective was to assess the potential genotoxic and mutagenic effects of the polluted water in three native fish species (Astyanax lacustris, Hypostomus ancistroides, and Rhamdia quelen) from Tarumã Microbasin, Upper Paraná River, Brazil.
View Article and Find Full Text PDFThe objective of this research was to evaluate land use and occupation in the landscape surrounding two neotropical streams of the Upper Paraná River in Brazil and to investigate if the water from these streams induces erythrocyte nuclear abnormalities in Astyanax lacustris. We observed that the areas surrounding the streams are predominantly used for agricultural purposes (73.46% of the total area), followed by buildings (15.
View Article and Find Full Text PDFThe present study evaluated mutagenic and genotoxic effects and metal accumulation in the liver and musculature of Pterygoplichthys ambrosetti and Prochilodus lineatus in the Amambai River, a tributary of the Upper Paraná River in Brazil. We also evaluated the potential for these fish species as environmental bioindicators and performed risk assessment for conservation purposes. We found that P.
View Article and Find Full Text PDFIn the present study, genotoxicity and mutagenicity were investigated in Tradescantia pallida exposed to vehicular traffic at different sites in a high-altitude tropical climate. During March, May, July, September, and November 2014, a comet assay and micronucleus bioassays were conducted on young inflorescences and leaves of T. pallida collected from twelve towns in the southern region of Mato Grosso do Sul with different amounts of vehicular traffic.
View Article and Find Full Text PDFToxicol Mech Methods
June 2017
Nuclear abnormalities (micronuclei and meta-nuclear changes) have been used as biomarkers to identify cell damages. As children are more vulnerable to the adverse effects of pollution when compared to adults, assessing genetic damage caused by environmental influences is of great interest. As such, the objective was to determine metanuclear (karyolysis, pycnosis, karyorrhexis, binucleated cells, chromosome bridges and micronuclei) in cells from the oral mucosa of children associated with the school environment, gender, exposure to cigarette smoke and vehicular traffic.
View Article and Find Full Text PDFInt J Environ Res Public Health
October 2016
Use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. However, this water is usually untreated, which makes it a vehicle for diseases. Thus, monitoring this water is necessary to ensure its integrity and quality.
View Article and Find Full Text PDFIn this study, the culture analysis of urine samples from patients hospitalized in the Central-West region of Brazil was performed, and the isolated microorganisms were phylogenetically identified as Trichosporon asahii. Maximum parsimony analysis of the IGS1 sequences revealed three novel genotypes that have not been described. The minimum inhibitory concentrations of the nine isolates identified were in the range of 0.
View Article and Find Full Text PDFTo characterize the effect of vehicular traffic on air quality, the micronuclei of Tradescantia pallida tetrads were counted. Young inflorescences of T. pallida (Rose) D.
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