Publications by authors named "Bruno Rocha Amando"
Antimicrobial and antibiofilm effect of promethazine on bacterial isolates from canine otitis externa: an in vitro study.
Microb Pathog
November 2024
Article Synopsis
- Otitis externa is a condition in dogs where the external ear canal becomes inflamed, usually due to bacterial infections, and this study focuses on testing promethazine's effectiveness against it.
- * The research evaluated how promethazine affects bacterial growth and biofilm formation over 120 hours, determining its minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentration (MBEC).
- * Results showed that promethazine significantly reduced biofilm growth and could be a promising treatment for recurring bacterial otitis in dogs, highlighting the benefits of repurposing existing drugs to combat antimicrobial resistance.
invasive candidiasis is considered a global health problem. In such cases, biofilm formation on implanted devices represents a therapeutic challenge and the presence of metabolically inactive persistent cells (PCs) in these communities increases their tolerance to fungicidal drugs. This study investigated the influence of amoxicillin, AMX; cefepime, CEF; gentamicin, GEN; amikacin, AMK; vancomycin, VAN; and ciprofloxacin, CIP; on the production of PCs in biofilms of bloodstream isolates.
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- The article investigates the use of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) as an efflux pump inhibitor to combat antimicrobial resistance and biofilm formation.
- It found that CCCP significantly reduces biofilm biomass and enhances the effectiveness of antibiotics against biofilms when used at certain concentrations (128 µM and 512 µM).
- CCCP also impacts the production of virulence factors, decreasing protease production and increasing siderophore release, indicating its potential as a therapeutic aid in managing infections related to biofilms.
This study evaluated the antimicrobial activity of promethazine against , and and its effect on the antimicrobial susceptibility of biofilms grown and on porcine heart valves. Promethazine was evaluated alone and in combination with vancomycin and oxacillin against spp. and vancomycin and ceftriaxone against in planktonic form and biofilms grown and .
View Article and Find Full Text PDFMicrobial biofilms are a natural adaptation of microorganisms, typically composed of multiple microbial species, exhibiting complex community organization and cooperation. Biofilm dynamics and their complex architecture are challenging for basic analyses, including the number of viable cells, biomass accumulation, biofilm morphology, among others. The methods used to study biofilms range from techniques to complex models.
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