Publications by authors named "Bruno Matuck"
The upper airway, particularly the nasal and oral mucosal epithelium, serves as a primary barrier for microbial interactions throughout life. Specialized niches like the anterior nares and the tooth are especially susceptible to dysbiosis and chronic inflammatory diseases. To investigate host-microbial interactions in mucosal epithelial cell types, we reanalyzed our single-cell RNA sequencing atlas of human oral mucosa, identifying polybacterial signatures (20% Gram-positive, 80% Gram-negative) within both epithelial- and stromal-resident cells.
View Article and Find Full Text PDFIdentifying cell types and states remains a time-consuming, error-prone challenge for spatial biology. While deep learning is increasingly used, it is difficult to generalize due to variability at the level of cells, neighborhoods, and niches in health and disease. To address this, we developed TACIT, an unsupervised algorithm for cell annotation using predefined signatures that operates without training data.
View Article and Find Full Text PDFIdentifying cell types and states remains a time-consuming and error-prone challenge for spatial biology. While deep learning is increasingly used, it is difficult to generalize due to variability at the level of cells, neighborhoods, and niches in health and disease. To address this, we developed TACIT, an unsupervised algorithm for cell annotation using predefined signatures that operates without training data, using unbiased thresholding to distinguish positive cells from background, focusing on relevant markers to identify ambiguous cells in multiomic assays.
View Article and Find Full Text PDFArticle Synopsis
- Sjögren's Disease (SjD) is an autoimmune disorder affecting salivary glands, but the cause and effective treatments are still unclear.
- Researchers used advanced techniques like single-cell and spatial transcriptomics to analyze both healthy and diseased salivary glands, revealing key differences in cellular composition.
- The study found that specific immune cells, particularly +CD8 T cells, are involved in damaging secretory cell types in SjD, highlighting the complex immune interactions that contribute to the disease's progression.
Article Synopsis
- - Emerging variants of SARS-CoV-2 may increase transmissibility, pathogenicity, and the risk of reinfection among individuals.
- - Antimicrobial photodynamic therapy (aPDT) is explored as an effective oral decontamination method for hospitalized COVID-19 patients to reduce microorganism load.
- - A case series analysis showed that aPDT significantly reduced the viral load of SARS-CoV-2 in the oral cavity shortly after treatment, indicating its potential as a decontamination strategy.
The ability of the new coronavirus SARS-CoV-2 to spread and contaminate is one of the determinants of the COVID-19 pandemic status. SARS-CoV-2 has been detected in saliva consistently, with similar sensitivity to that observed in nasopharyngeal swabs. We conducted ultrasound-guided postmortem biopsies in COVID-19 fatal cases.
View Article and Find Full Text PDFOral focal mucinosis (OFM) is an uncommon, asymptomatic, submucosal, slow-growing nodule representing a counterpart of the cutaneous focal mucinosis (CFM). OFM has a female predilection with the highest prevalence in the fifth decade of life. About 68% of OFMs occur in the gingiva and 14% in the palate.
View Article and Find Full Text PDFPurpose: We aimed to compare the histomorphological features of oral lymphoepithelial cysts (OLC) in different locations of the oral cavity and to verify the association between oral lymphoepithelial cysts and subgemmal neurogenous plaque (SNP) on biopsies from the lateral border of the tongue.
Methods: All cases diagnosed as OLC from the Oral Pathology Service at the School of Dentistry/University of São Paulo were retrieved. For all the cysts located in the tongue, their association to SNP was analyzed.