Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set.

Background: The efficiency of isolation and purification of the viral genome is a critical step to the accuracy and reliability of RT-qPCR to detect SARS-CoV-2. However, COVID-19 testing laboratories were overwhelmed by a surge in diagnostic demand that affected supply chains especially in low and middle-income facilities.

Objectives: Thus, this study compares the performance of alternative methods to extraction and purification of viral RNA in samples of patients diagnosed with COVID-19.

Hematological and immunological effects of stress of air traffic controllers in northeastern Brazil.

Background: Several studies have shown that stress and emotional reactions can affect immune responses in animals and humans.

Objective: The aim of this study was to evaluate hematological and immunological effects of stress on air traffic controllers.

Methods: Thirty air traffic controllers and 15 aeronautical information service operators were evaluated.