Ultra-sensitive detection of Mycobacterium leprae: DNA extraction and PCR assays.

Leprosy urgently needs a precise and early diagnostic tool. The sensitivity of the direct (bacilli staining, Mycobacterium leprae DNA) and indirect (antibody levels, T cell assays) diagnostics methods vary based on the clinical form. Recently, PCR-based M.

Evaluation of dried blood spot samples for hepatitis C virus detection and quantification.

Background: Dried blood spots (DBS) could be an excellent alternative for HCV diagnosis, since it is less invasive and can be stored and transported without refrigeration.

Objectives: The aim of this study was to optimize quantitative and qualitative methods for HCV detection in DBS.

Study Design: DBS and serum samples were collected from 99 subjects (59 anti-HCV/HCV RNA positive and 40 seronegative samples).