The Stilbene Synthase Family in : A Genome-Wide Study and Functional Characterization in Response to Stress.

Peanut () and its wild relatives are among the few species that naturally synthesize resveratrol, a well-known stilbenoid phytoalexin that plays a crucial role in plant defense against biotic and abiotic stresses. Resveratrol has received considerable attention due to its health benefits, such as preventing and treating various human diseases and disorders. Chalcone (CHS) and Stilbene (STS) Synthases are plant-specific type III Polyketide Synthases (PKSs) that share the same substrates and are key branch enzymes in the biosynthesis of flavonoids and stilbenoids, respectively.

A novel soybean hairy root system for gene functional validation.

Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions.

Engineering Resistance against Using a Truncated NLR (TNx) and a Defense-Priming Gene.

The association of both cell-surface PRRs (Pattern Recognition Receptors) and intracellular receptor NLRs (Nucleotide-Binding Leucine-Rich Repeat) in engineered plants have the potential to activate strong defenses against a broad range of pathogens. Here, we describe the identification, characterization, and in planta functional analysis of a novel truncated NLR (TNx) gene from the wild species (), with a protein structure lacking the C-terminal LRR (Leucine Rich Repeat) domain involved in pathogen perception. Overexpression of in tobacco plants led to a significant reduction in infection caused by , with a further reduction in pyramid lines containing an expansin-like B gene () potentially involved in defense priming.

Ex vitro hairy root induction in detached peanut leaves for plant-nematode interaction studies.

Background: Peanut () production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) that causes yield losses worldwide. Transcriptome studies of wild species, which harbor resistance to a number of pests and diseases, disclosed several candidate genes for resistance. Peanut is recalcitrant to genetic transformation, so the use of -derived hairy roots emerged as an alternative for in-root functional characterization of these candidate genes.

Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene.

Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A.