Wound fluid sampling methods and analysis of cytokine mRNA expression in ulcers from patients with diabetes mellitus.

The development of diabetic foot ulcers is a common and severe complication of diabetes that can significantly affect quality of life. The physiological healing cascade does not progress tissue repair in diabetic foot ulcerations in a timely manner. Serum markers from foot ulcers have been used to characterize the healing process of the diabetic foot using various collection techniques.

Clinical nursing care protocol for convalescent plasma transfusion in patients with COVID-19.

Introduction: The treatment of COVID-19 is still challenge. So convalescent plasma can be an important alternative of treatment. Protocols with nursing care during infusion is very important to guide an effective and safety care.

Microbiological identification and resistance profile of microorganisms in pressure injuries after the use of polyhexamethylene biguanide: a series of fourteen cases.

Introduction: Colonization of a pressure injury with microorganisms can negatively affect wound healing. Thus, it is necessary to evaluate which products best facilitate wound healing.

Objective: This case series evaluated the effectiveness of the antimicrobial polyhexamethylene biguanide (PHMB) on microorganisms in pressure injuries.

Does pH Influence the Bacterial Profile of Chronic Lesions? An Analysis of Venous Ulcer Samples.

Objective: To analyze the pH of venous ulcers and their relationship with the quantitative and qualitative bacterial profile of the wounds.

Methods: Cross-sectional study carried out through data collection and microbiologic analyses of samples obtained from 35 venous ulcers. Investigators performed pH measurement with indicator strips and collected biologic material using swabs.

Blood Cell Count and the Presence or Absence of Infection in Venous Ulcers Treated With Platelet-Rich Plasma.

Introduction: In general, chronic wounds are colonized by bacteria; however, when microorganisms start to multiply at higher levels, wounds can become infected, causing prolongation of the inflammatory phase and retardation of collagen synthesis and epithelialization.

Objective: The objective of this study was to evaluate the presence of infection in venous ulcers after 12 weeks of treatment with autologous platelet-rich plasma (PRP) and determine global white blood cell counts.

Materials And Methods: This case series study involved a sequential sample of 17 patients with venous ulcers treated with PRP for 12 weeks.

Clinical and Microbiological Outcomes Associated With Use of Platelet-Rich Plasma in Chronic Venous Leg Uclers: A Randomized Controlled Trial.

Purpose: To evaluate the susceptibility profiles of Staphylococcus aureus and Pseudomonas aeruginosa strains identified in chronic venous ulcers treated with platelet-rich plasma (PRP) and petrolatum gauze or petrolatum gauze alone and to quantitatively evaluate the bacterial load and biofilm-forming capacities of the detected S. aureus and P. aeruginosa strains.

Ciprofloxacin-Resistant Kerstersia gyiorum Isolated From a Chronic Wound in Brazil: A Case Report.

Unlabelled: The presence of Kerstersia gyiorum in lower leg wounds has been reported in case studies from several countries.

Objective: This study evaluated the antimicrobial susceptibility profile of K gyiorum isolated from a chronic wound.

Methods: An 85-year-old woman with chronic venous insufficiency presented to an intermediate care unit in Niteroi City, Rio de Janeiro, Brazil, with an instep chronic wound of 14 cm² with wound duration of 6 months.

Development of an Educational Protocol Based on a Nursing Team's Knowledge of Pulse Therapy in Adolescents in Brazil.

The aim of this study was to assess the knowledge of nursing teams that care for hospitalized adolescents undergoing glucocorticoid infusion and to develop an educational protocol for nursing care during pulse therapy. This descriptive, exploratory study with a qualitative approach was developed in a unit specializing in adolescent health at a university hospital in the state of Rio de Janeiro, Brazil. The instrument used for data collection was a semistructured interview conducted in a private room on the unit.

Monitoring and Molecular Characterization of Staphylococcus aureus Isolated from Chronic Wounds.

Objective: Researchers analyzed chronic wounds treated with 2% hydrogel to determine whether the presence of methicillin-resistant Staphylococcus aureus (MRSA) is related to the presence of clinical signs of infection.

Methods: Thirty-five patients were recruited for this descriptive study using a quantitative approach. Staphylococcus aureus was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Prevalence, Antimicrobial Susceptibility, and Clonal Diversity of Pseudomonas aeruginosa in Chronic Wounds.

Purpose: Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility of P. aeruginosa strains to various antimicrobials, (3) detect the presence of virulence factors exoenzyme S (exoS) and exoenzyme U (exoU) in P. aeruginosa strains, and (4) evaluate wound colonization by P.