Exploring Botryosphaeran, a (1 → 3)(1 → 6)-β-D-Glucan, as a Matrix for the Stabilization of Laccase from Pleurotus ostreatus Florida onto a Zinc Oxide Quantum Dots Platform for the Electrochemical Determination of 2,6-Dimethoxyphenol.

This work describes the development of a novel biosensor obtained by immobilizing laccase from Pleurotus ostreatus Florida onto a glassy carbon electrode platform modified with zinc oxide quantum dots. For enzyme immobilization, the exopolysaccharide botryosphaeran from Botryosphaeria rhodina MAMB-05 was used. Although both biomaterials are from different fungal sources, laccase immobilization was guaranteed, which was demonstrated by the excellent stability of the fabricated biosensor device for the voltammetric determination of 2,6-dimethoxyphenol (2,6-DMP).

Assessment of the use of boron-doped diamond electrode for highly sensitive voltammetric determination of the azo-dye carmoisine E-122 in food and environmental matrices.

A new protocol for the analysis of the azo-dye carmoisine (CMS) is presented by coupling differential pulse voltammetry (DPV) with a cathodically pretreated boron-doped diamond electrode (CPT-BDDE), in phosphate buffer solution (pH 2.0). The CMS presented diffusion-controlled oxidation and reduction peaks at +0.

Covalent attachment of laccase to carboxymethyl-botryosphaeran in aqueous solution for the construction of a voltammetric biosensor to quantify quercetin.

Laccase from Botryosphaeria rhodina MAMB-05 was covalently immobilized on carboxymethyl-botryosphaeran by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) in aqueous solution. This approach was employed to fabricate a novel laccase-based biosensor to electrochemically quantify quercetin (QCT), using a simple carbon black paste electrode as a transducer. The proposed biosensor was characterized by electrochemical impedance spectroscopy and Nyquist plots were used to evaluate the immobilization of the enzyme.