Goondicones A-H: Spiro-Isoindolinone Heartworm Anthelmintics from an Australian Pasture-Soil-Derived sp.

Background/objectives: There is an urgent need for new and improved anthelmintics that are not constrained by existing resistance pathways and that can safeguard the health and welfare of animals.

Methods: An integrated platform of chemical, bioassay, and cultivation profiling applied to a library of microbes isolated from Australian livestock pasture soil was used to detect and guide the production, isolation, characterization, identification, and evaluation of new natural products with anthelmintic properties.

Results: A global natural products social (GNPS) molecular network analysis of 110 Australian pasture-soil-derived microbial extracts prioritized for antiparasitic activity identified unique molecular families in the extract of sp.

Goondomycins A-H: Carbocyclic -Polyketides from an Australian Pasture with Selective Activity against .

A bioassay-guided chemical investigation of an Australian pasture-soil-derived sp. yielded the rare carbocyclic -polyketide kendomycin () along with a series of new analogues, goondomycins A-H (-), featuring unprecedented carbo/heterocyclic scaffolds and chromophores, with structures assigned by detailed spectroscopic analysis, chemical and biochemical transformations, and biosynthetic considerations. Goondomycins B () and F () are noteworthy in being potent motility inhibitors of heartworm microfilaria (EC 0.

Goondolinones A and B: Terpenyl-quinolin-4(1)-ones from an Australian Volcanic Crater Soil-Derived sp., with Selective Activity against (Heartworm).

A chemical investigation of the Australian pasture soil-derived sp. S4S-00245B09, supported by chemical, bioactivity and cultivation profiling, yielded a new class of terpenyl-quinolin-4(1)-ones, goondolinones A and B ( and ), featuring an unprecedented fused seven-membered ether. Structures were assigned to and on the basis of detailed spectroscopic analysis, including X-ray analysis of , and biosynthetic considerations.

Healthcare Resource Utilization Among Patients With Agitation in Alzheimer Dementia.

Agitation in Alzheimer dementia is common, but the associated healthcare burden remains unclear. This retrospective analysis evaluated baseline characteristics, healthcare resource utilization, and costs among patients with agitation in Alzheimer dementia and those without agitation in Alzheimer dementia. Medicare beneficiaries from 100% of the Medicare Fee-for-Service claims database (2009-2016) with 2 or more claims 30 or more days apart for both Alzheimer's disease and dementia and continuous enrollment with medical/pharmacy coverage for 6 months before and 12 months after the index diagnosis were included.

Goondapyrones A-J: Polyketide α and γ Pyrone Anthelmintics from an Australian Soil-Derived sp.

An investigation of ×19 soil samples collected under the auspices of the Australian citizen science initiative, Soils for Science, returned ×559 chemically dereplicated microbial isolates, of which ×54 exhibited noteworthy anthelmintic activity against either the heartworm microfilaria and/or the gastrointestinal parasite L1-L3 larvae. Chemical (GNPS and UPLC-DAD) and cultivation (MATRIX) profiling prompted a detailed chemical investigation of sp. S4S-00196A10, which yielded new anthelmintic polyketide goondapyrones A-J (-), together with the known actinopyrones A () and C ().

Simple and Accurate Representation of Cumulative Nighttime Leaf Respiratory CO Efflux.

Leaf respiratory carbon loss decreases independent of temperature as the night progresses. Detailed nighttime measurements needed to quantify cumulative respiratory carbon loss at night are challenging under both lab and field conditions. We provide a simple yet accurate approach to represent variation in nighttime temperature-independent leaf respiratory CO efflux in environments with both stable and fluctuating temperatures, which requires no detailed measurements throughout the night.

Australian Marine and Terrestrial -Derived Surugamides, and Synthetic Analogs, and Their Ability to Inhibit (Heartworm) Motility.

A bioassay-guided chemical investigation of a bacterium, sp. CMB-MRB032, isolated from sheep feces collected near Bathurst, Victoria, Australia, yielded the known polyketide antimycins A4a () and A2a () as potent inhibitors of (heartworm) microfilaria (mf) motility (EC 0.0013-0.

Systematic Literature Review of Studies Reporting Measures of Functional Outcome or Quality of Life in People with Negative Symptoms of Schizophrenia.

Aim: Negative symptoms of schizophrenia (NSS) have been linked with poor functional outcomes. A literature review was performed to identify instruments used to assess functional outcomes and quality of life in clinical trials and observational studies conducted in groups of people with NSS.

Methods: Literature search strings were designed using Medical Subject Headings combined with free-text terms and searches were performed using the PubMed, Embase and the Cochrane Library databases.

Strong acclimation effect of temperature and humidity on heat tolerance of the Arctic collembolan Megaphorura arctica.

The Arctic is a highly variable environment in which extreme daily and seasonal temperature fluctuations can occur. With climate change, an increase in the occurrence of extreme high temperatures and drought events is expected. While the effects of cold and dehydration stress on polar arthropods are well studied in combination, little is known about how these species respond to the combined effects of heat and dehydration stress.

Effects of Social Facilitation and Introduction Methods for Cattle on Virtual Fence Adaptation.

Agricultural industries rely on physical fences to manage livestock. However, these present practical, financial, and ecological challenges, which may be solved using virtual fencing. This study aimed to identify how experienced cattle through social facilitation and the introduction method influence inexperienced cattle.

Instantaneous Q of night-time leaf respiratory CO efflux - measurement and analytical protocol considerations.

The temperature sensitivity (e.g. Q) of night-time leaf respiratory CO efflux (R) is a fundamental aspect of leaf physiology.

Measured leaf dark respiratory CO -release is not controlled by stomatal conductance.

Leaf dark respiratory CO -release (R ) is, according to some literature, dependent on the rate of leaf transpiration. If this is true, then at a given vapor pressure deficit, the leaf stomatal conductance (g ) will be expected to be a controlling factor of measured R at any given time. We artificially lowered leaf g by applying abscisic acid (ABA).

eDNA Metabarcoding- and Microscopic Analysis for Diet Determination in Waterfowl, a Comparative Study in Vejlerne, Denmark.

Understanding diets and structural food webs are keys to the apprehension of ecological communities, upon which conservation and management biology are based. The understanding of grazing and habitat choice for waterfowl is one of the most important topics for avian ecologists today and can, to some degree, be answered by dietary analysis. Droppings collected from four waterfowl, the Eurasian wigeon (), Greylag goose (), pink-footed goose () and Barnacle goose () in Vejlerne (Denmark), were analysed microscopically and through eDNA metabarcoding with the use of next generation sequencing (NGS) to accumulate knowledge about the diet of these waterfowl.

Improved description of terrestrial habitat types by including microbial communities as indicators.

Soils host diverse communities of microorganisms essential for ecosystem functions and soil health. Despite their importance, microorganisms are not covered by legislation protecting biodiversity or habitats, such as the Habitats Directive. Advances in molecular methods have caused breakthroughs in microbial community analysis, and recent studies have shown that parts of the communities are habitat-specific.

The Effectiveness of Virtual Fencing of Bull Calves in a Holistic Grazing System.

Large grazers are essential for nature conservation. In order to prevent grazers from moving to unintended areas, it may be necessary to keep them inside enclosures. Physical fences present a number of problems, such as fragmenting the landscape.

Activity-dependent nocturnal decrease in leaf respiration.

Nocturnal decrease in leaf respiratory CO-release measured at constant temperature within a plant species is correlated with the level of respiratory activity at onset of the dark period.

Depression as a major component of a gait disorder-Successful multimodal treatment including electroconvulsive therapy : A case report.

This case report describes an 82-year-old patient who was treated in a gerontological psychiatric ward due to a multifactorial gait disorder with falls. The main component of the gait disorder was depression, which was accompanied by a pronounced fear of falling. Other factors were polyneuropathy, gonarthrosis and an exercise deficit after previous inactivity.

Nocturnal plant respiration is under strong non-temperature control.

Most biological rates depend on the rate of respiration. Temperature variation is typically considered the main driver of daily plant respiration rates, assuming a constant daily respiration rate at a set temperature. Here, we show empirical data from 31 species from temperate and tropical biomes to demonstrate that the rate of plant respiration at a constant temperature decreases monotonically with time through the night, on average by 25% after 8 h of darkness.