Is there sufficient evidence to inform personal protective equipment choices for healthcare workers caring for patients with viral hemorrhagic fevers?

Background: Ugandan health authorities declared an outbreak of Ebola disease (EBOD), caused by the Sudan virus, in September 2022. A rapid review was conducted to update the Public Health Agency of Canada's guidelines for infection prevention and control measures for EBOD in healthcare settings to prepare for potential introduction of cases.

Objective: Summarize the available evidence on personal protective equipment (PPE) use by healthcare workers (HCWs) to prevent exposure to and transmission of viral hemorrhagic fevers (VHFs), including Ebola virus.

New weapons explosive exhibits persistent toxicity in plants.

Explosives are widespread, toxic and persistent environmental pollutants. 2,4-Dinitroanisole (DNAN) is being phased in to replace 2,4,6-trinitrotoluene (TNT) in munitions. Here we demonstrate that only low levels of DNAN are detoxified in Arabidopsis, leaving it to remain as a substrate for monodehydroascorbate reductase 6 mediated chronic phytotoxicity.

Quantifying and Learning Static Vs. Dynamic Information in Deep Spatiotemporal Networks.

There is limited understanding of the information captured by deep spatiotemporal models in their intermediate representations. For example, while evidence suggests that action recognition algorithms are heavily influenced by visual appearance in single frames, no quantitative methodology exists for evaluating such static bias in the latent representation compared to bias toward dynamics. We tackle this challenge by proposing an approach for quantifying the static and dynamic biases of any spatiotemporal model, and apply our approach to three tasks, action recognition, automatic video object segmentation (AVOS) and video instance segmentation (VIS).

A mechanism for slow rhythms in coordinated pancreatic islet activity.

Insulin levels in the blood oscillate with a variety of periods, including rapid (5-10 min), ultradian (50-120 min), and circadian (24 h). Oscillations of insulin are beneficial for lowering blood glucose and disrupted rhythms are found in people with type 2 diabetes and their close relatives. These in vivo secretion dynamics imply that the oscillatory activity of individual islets of Langerhans are synchronized, although the mechanism for this is not known.

Controlling Gold Nanoparticle Synthesis and Size for Catalysis.

Gold nanoparticles (Au-NPs) are used as catalysts for a diverse range of industrial applications. Currently, Au-NPs are synthesized chemically, but studies have shown that plants fed Au deposit, this element naturally as NPs within their tissues. The resulting plant material can be used to make biomass-derived catalysts.

NO1 tailors its secretome for different lignocellulosic substrates.

NO1 is a plant biomass-degrading ascomycete with a propensity to target the most recalcitrant components of lignocellulose. Here we applied proteomics and activity-based protein profiling (ABPP) to investigate the ability of NO1 to tailor its secretome for growth on different lignocellulosic substrates. Proteomic analysis of soluble and insoluble culture fractions following the growth of NO1 on six lignocellulosic substrates highlights the adaptability of the response of the NO1 secretome to different substrates.

Biotinylated Photocleavable Semiconductor Colloidal Quantum Dot Supraparticle Microlaser.

Luminescent supraparticles of colloidal semiconductor nanocrystals can act as microscopic lasers and are hugely attractive for biosensing, imaging, and drug delivery. However, biointerfacing these to increase functionality while retaining their main optical properties remains an unresolved challenge. Here, we propose and demonstrate red-emitting, silica-coated CdSSe/ZnS colloidal quantum dot supraparticles functionalized with a biotinylated photocleavable ligand.

Using implementation science to decrease variation and high opioid administration in a surgical ICU.

Background: High doses and prolonged duration of opioids are associated with tolerance, dependence, and increased mortality. Unfortunately, despite recent efforts to curb outpatient opioid prescribing because of the ongoing epidemic, utilization remains high in the intensive care setting, with intubated patients commonly receiving infusions with a potency much higher than doses required to achieve pain control. We attempted to use implementation science techniques to monitor and reduce excessive opioid prescribing in ventilated patients in our surgical intensive care unit (SICU).

Higher Doses of Calcium Associated With Survival in Trauma Patients.

Introduction: Calcium is required for coagulation, cardiac output, and peripheral vascular resistance. Between 85% and 94% of trauma patients treated with massive blood transfusion develop hypocalcemia. The aim of this study is to evaluate the relationship between increased intravenous calcium administration during massive transfusion and improved survival of trauma patients.

A new species of Cirolana Leach, 1818 (Isopoda, Cymothoidea, Cirolanidae), from Havelock Island, Andaman Sea, northern Indian Ocean.

Cirolana swaraj sp. nov. was collected from brackish-water Neil Lagoon on Havelock Island, South Andaman and is described and illustrated in detail.

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh.

Realising a fully circular bioeconomy requires the valorisation of lignocellulosic biomass. Cellulose is the most attractive component of lignocellulose but depolymerisation is inefficient, expensive and resource intensive requiring substantial volumes of potable water. Seawater is an attractive prospective replacement, however seawater tolerant enzymes are required for the development of seawater-based biorefineries.

DNA barcoding of the supergiant isopods from Lowry & Dempsey, 2006 (Cirolanidae) and a molecular biology comparison of Kou, Chen & Li, 2017.

DNA was extracted from tissue samples from specimens of newly-collected from Queensland and subsequently the COI and 16S rRNA sequences were successfully cloned. The holotype of was also sampled for COI only. Comparison of the sequences showed that, for the COI sequences, and have more than 59 different DNA positions amongst 596 known reading sequences.

Cassidinidea andamanensis sp. nov., a new species of intertidal Sphaeromatidae (Crustacea: Isopoda) from the Andaman Islands, northern Indian Ocean.

Cassidinidea andamanensis sp. nov. was collected from intertidal habitats on the coast of South Andaman, Andaman Islands, India and is described and illustrated in detail.

A new species of deep-sea Booralana Bruce, 1986 (Crustacea: Isopoda: Cirolanidae) from The Bahamas, Western North Atlantic.

Booralana nickorum sp. nov. is described from the deep-water slope of the Exuma Sound, The Bahamas, from depths of 540 to 560 metres.

Optimized configuration for liquid crystal Stokes polarimeters in the presence of fast-axis orientation errors.

We present an optimal configuration for Stokes polarimeters based on liquid crystal variable retarders, with the minimum number of measurements. Due to the inherent variations of the director orientation of the liquid crystal molecules, we propose a configuration that minimizes the sensibility of the polarimeter to fast-axis variations. For the optimization we consider a scheme that maximizes the volume of a tetrahedron inscribed in the Poincare sphere, to address additive and Poisson noise, with one of the vertices invariant to changes in the axis positions.

Polarimetric measurement of non-depolarizing optical systems.

The use of polarization measurements has become more common in recent years, as it gives more information than pure intensity measurements. Polarimetric components such as fixed or variable retarders and polarizers must be included in optical systems to obtain the polarization parameters required, and in many cases the optical system also includes other components such as relay and/or imaging optical systems. In this work we present a simple and robust method for the polarimetric characterization of non-depolarizing polarization components and other optical elements in the system, which does not require a full polarimeter.

Human sperm rotate with a conserved direction during free swimming in four dimensions.

Head rotation in human spermatozoa is essential for different swimming modes and fertilisation, as it links the molecular workings of the flagellar beat with sperm motion in three-dimensional (3D) space over time. Determining the direction of head rotation has been hindered by the symmetry and translucent nature of the sperm head, and by the fast 3D motion driven by the helical flagellar beat. Analysis has been mostly restricted to two-dimensional (2D) single focal plane image analysis, which enables tracking of head centre position but not tracking of head rotation.

A bioinformatic workflow for in silico secretome prediction with the lignocellulose degrading ascomycete fungus Parascedosporium putredinis NO1.

The increasing availability of microbial genome sequences provides a reservoir of information for the identification of new microbial enzymes. Genes encoding proteins engaged in extracellular processes are of particular interest as these mediate the interactions microbes have with their environments. However, proteomic analysis of secretomes is challenging and often captures intracellular proteins released through cell death and lysis.

The Impact of Acute Systemic Inflammation Secondary to Oesophagectomy and Anastomotic Leak on Computed Tomography Body Composition Analyses.

This study aimed to longitudinally assess CT body composition analyses in patients who experienced anastomotic leak post-oesophagectomy. Consecutive patients, between 1 January 2012 and 1 January 2022 were identified from a prospectively maintained database. Changes in computed tomography (CT) body composition at the third lumbar vertebral level (remote from the site of complication) were assessed across four time points where available: staging, pre-operative/post-neoadjuvant treatment, post-leak, and late follow-up.

A review of the family Cymothoidae (Isopoda: Cymothooidea) infesting marine fishes from Malaysian waters, with new host and geographical records.

A checklist of parasitic cymothoids from Malaysian waters is presented based on available literature and material collected from 2010 to 2020. Most of the collected specimens were recorded from waters of Terengganu, east coast of Peninsular Malaysia (facing the South China Sea), whereas literature records were included from Sarawak, along the Miri coast of northwest Borneo. The checklist comprises 19 species in ten genera, seven of which are new records from Malaysia: Anilocra nemipteri Bruce, 1987; Ceratothoa barracuda Martin, Bruce & Nowak, 2015; Ceratothoa carinata (Bianconi, 1869); Cymothoa epimerica Avdeev, 1979; Elthusa sigani Bruce, 1990; Joryma engraulidis (Barnard, 1936) and Renocila richardsonae Williams & Bunkley-Williams, 1992.

Moving the needle on time to resuscitation: An EAST prospective multicenter study of vascular access in hypotensive injured patients using trauma video review.

Background: Vascular access in hypotensive trauma patients is challenging. Little evidence exists on the time required and success rates of vascular access types. We hypothesized that intraosseous (IO) access would be faster and more successful than peripheral intravenous (PIV) and central venous catheter (CVC) access in hypotensive patients.

MicroLED biosensor with colloidal quantum dots and smartphone detection.

A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles.