The histology and ultrastructure of small endometriotic lesions were studied in 19 patients before and after hormonal therapy with the anti-progesterone steroid R 2323 (Gestrinone). Histologic results demonstrate that treatment of endometriosis with this steroid does not result in complete elimination of the endometriotic foci, although glandular proliferation and secretion are arrested in most implants. The ultrastructural results indicate that this inhibition of proliferation and secretion is related to an enhanced activity of the lysosomal system in the epithelial cells of some endometriotic foci.
View Article and Find Full Text PDFOne of the most important and fundamental prerequisites for successful microsurgery is serious and adequate training. This article presents the effect of microsurgical training on the functional end result of reanastomosis in the rabbit fallopian tube and evaluates the appropriate duration of laboratory training. In 50 New Zealand White female rabbits a microsurgical reanastomosis of the fallopian tube in its isthmic portion was performed.
View Article and Find Full Text PDFThe restoration of normal tubo-ovarian function was investigated in a training programme using oviduct anastomosis in the rabbit as an experimental model. Ovulation rate, pregnancy rate, nidation index, implantation failure index and degree of adhesions were used as parameters. More than 100 anastomoses had to be performed to reach a perfect degree of atraumatic, function-restoring surgery.
View Article and Find Full Text PDFAn ultrastructural review of the cyclic changes of endometrial surface and glandular epithelial cells, stromal cells and the stromal microvasculature is presented. Endometrial biopsies were collected from infertile patients with endometriosis or tubal dysfunction. During the proliferative phase, the ergastoplasm and Golgi apparatus of surface and glandular epithelial cells become well-developed, while increasing numbers of mitochondria are seen.
View Article and Find Full Text PDFAnn N Y Acad Sci
August 1985
Clumps of corona cells were separated from oocytes at the moment of fertilization control and were subsequently processed and cut into 1-mu sections to study their general morphologic patterns. This study revealed an extensive phagocytosis of spermatozoa by corona cells. Clumps of mural granulosa cells of comparable size, recovered from follicular aspirates and incubated with spermatozoa, did not show the same extent of phagocytotic activity.
View Article and Find Full Text PDFIt is only relatively recently that attention has been directed to studies of the uterine side of the placenta to look for possible defects that might explain otherwise inexplicable pregnancy complications. Preeclampsia and intrauterine fetal growth retardation are two such disorders, in which new information has come to light by the study of placental bed biopsies and occasional cesarean hysterectomy specimens. It will be less easy to apply these techniques to such problems as spontaneous abortion and antepartum hemorrhage, but reemphasizing what should be the self-evident importance of the establishment and development of the uteroplacental blood supply might help reorient thinking about these and other important complications of pregnancy.
View Article and Find Full Text PDFEur J Obstet Gynecol Reprod Biol
December 1984
In 36 patients with laparoscopically diagnosed endometriosis, biopsies were taken from different areas of the pelvic peritoneum bearing foci of endometriosis. The biopsies were studied by scanning electron microscopy and by light microscopy. Combined use of these techniques resulted in the differentiation of three topographically and morphologically different types of endometriotic lesions: intraperitoneal endometriotic polyps with no glandular openings but associated with deeper endometriotic glands and stroma; intraperitoneal endometriotic foci with surface epithelium, glands, and stroma; and retroperitoneal small lesions with few glands and scant stroma.
View Article and Find Full Text PDFFrom January 1973 to December 1980, 176 infertile women with endometriosis were treated with atraumatic and microsurgical techniques. None of these patients received preoperative or postoperative antigonadotropin therapy. The degree of endometriosis in these patients was classified according to the scaling point system of The American Fertility Society.
View Article and Find Full Text PDFEur J Obstet Gynecol Reprod Biol
September 1984
In 137 oviducts of New Zealand white rabbits different kinds of occlusion techniques were tested to induce hydrosalpinges. In 99 oviducts ligatured or clipped both at the fimbrial end and at the ampullary-isthmic junction 67 hydrosalpinges were achieved, whilst in 34 oviducts ligatured or clipped only at the fimbrial end one hydrosalpinx occurred. Most severe histological changes were seen after double clipping.
View Article and Find Full Text PDFEur J Obstet Gynecol Reprod Biol
September 1984
Twenty-five artificially induced hydrosalpinges were studied in New Zealand white rabbits. Hydrosalpinges had been created after 2-16 wk occlusion of both the fimbrial end and the ampullary isthmic junction (A.I.
View Article and Find Full Text PDFAfter an electrically induced pseudopregnancy in rats in which deciduomas were produced by an intraluminal oil injection, the decidual tissue was studied morphologically on days 7, 10 and 13. A constant and dynamic wave of mitotic figures was found, which started on day 7 in the antimesometrial decidua, moving to the mesometrial decidua on day 10 and finally to the mesometrial triangle area on day 13 of pseudopregnancy. This and other morphological changes were compared with those found in pseudopregnant rats with streptozotocin-induced diabetes.
View Article and Find Full Text PDFIn an experimental animal study, two different microsurgical techniques of vasoepididymostomy to the caput epididymidis are compared: an end-to-side anastomosis with the epididymal tubule and an end-to-end anastomosis with a section of a cluster of the epididymal tubule. The patency rates are respectively 50 per cent and 26.3 per cent, as controlled by deferentography and histology.
View Article and Find Full Text PDFMorphometric and statistical techniques were used to assess the relation of myometrial interstitial trophoblast to the uteroplacental vasculature in 27 intact hysterectomy specimens ranging from 8 to 18 weeks' gestation. It was found that the volume density of cytotrophoblast in the myometrium and in particular the proximity of such trophoblast to the placental bed spiral arteries correlated significantly with morphological alterations in these vessels. The changes included swelling of endothelium, hypertrophy of individual medial smooth muscle cells, and oedema and disruption of the architecture of the vessel wall as a time-related continuum.
View Article and Find Full Text PDFBr J Obstet Gynaecol
August 1983
Multiple tubal biopsies from 21 patients with thin-walled dilated hydrosalpinges and from 12 patients with thick-walled fibrous hydrosalpinges were studied. All the biopsies were examined by light microscopy and scanning electron microscopy and the ciliated surface area at multiple locations measured by planimetry. These biopsies were compared with control biopsies taken from 13 normal women at varying stages of the menstrual cycle.
View Article and Find Full Text PDFFallopian tubes containing a gestation are frequently normal on macroscopical and gross histological examination. Scanning electron microscopy and light microscopy studies of tubal biopsies taken from five groups of women showed marked differences in the ciliated surface which was measured planimetrically on photographs. The proportion of ciliated cells was significantly lower in biopsies taken from 25 women with tubal pregnancies compared with that in biopsies from seven women with intrauterine pregnancies at the same gestation.
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