[Isolation and detection of antibodies against nucleotide sequences. A study with oligonucleotide pApCpApC].

New methods for preparation and detection of antibodies to nucleotide sequences were developed. An efficient method for conjugation of oligonucleotides to proteins based on the activation of 5'-terminal oligonucleotide phosphate with dimethylaminopyridine was employed. Oligonucleotide pApCpApC was conjugated to bovine serum albumin with a 90% yield.

[Complementary addressed modification of single-stranded DNA using a [Fe-EDTA] derivative of oligonucleotide].

A one-tube synthesis scheme for coupling EDTA residue to the 5'-terminus of unprotected oligonucleotides via propylenediamine linker is described. The EDTA derivative of oligonucleotide d(pTGACCCTCTTCCC)A forms a kinetically stable complex with Fe2+ ion. In the presence of ascorbic acid with O2 limiting, this complex modifies single-stranded DNA (a 302-mer) in a site-specific way near the target complementary nucleotide sequence.

[Construction of DNA-probes and their immunochemical detection using nonradioactive hybridization tests].

Simple and efficient chemical approaches to preparation of DNA probes carrying 2,4-dinitrophenyl, dansyl or biotin residues were developed. The residues were introduced using following DNA derivatization procedures: a) transamination of cytidine residues with O-(4-aminobutyl)hydroxylamine; b) mercuration of pyrimidine residues followed by beta-mercaptoethanol modification. It was shown that 2,4-dinitrophenyl-containing DNA probes can be used for nonradioactive hybridization detection of nucleic acids.