Epigenome-wide association study of peripheral immune cell populations in Parkinson's disease.

Understanding the contribution of immune mechanisms to Parkinson's disease pathogenesis is an important challenge, potentially of major therapeutic implications. To further elucidate the involvement of peripheral immune cells, we studied epigenome-wide DNA methylation in isolated populations of CD14 monocytes, CD19 B cells, CD4 T cells, and CD8 T cells from Parkinson's disease patients and healthy control participants. We included 25 patients with a maximum five years of disease duration and 25 controls, and isolated four immune cell populations from each fresh blood sample.

Quantitative proteomics reveals protein dysregulation during T cell activation in multiple sclerosis patients compared to healthy controls.

Background: Multiple sclerosis (MS) is an autoimmune, neurodegenerative disorder with a strong genetic component that acts in a complex interaction with environmental factors for disease development. CD4 T cells are pivotal players in MS pathogenesis, where peripherally activated T cells migrate to the central nervous system leading to demyelination and axonal degeneration. Through a proteomic approach, we aim at identifying dysregulated pathways in activated T cells from MS patients as compared to healthy controls.

Global DNA methylation changes in treated and untreated MS patients measured over time.

Multiple sclerosis (MS) is an autoimmune, neurological disease. We investigated genome-wide DNA methylation profiles of CD4 and CD8 T cells from MS patients and healthy controls at baseline and a follow-up visit. Patients were all treatment-naïve at baseline, and either on treatment or remained untreated at the follow-up visit.

CD8 T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Background: Genetic and clinical observations have indicated T cells are involved in MS pathology. There is little insight in how T cells are involved and whether or not these can be used as markers for MS.

Objectives: Analysis of the gene expression profiles of circulating CD8 T cells of MS patients compared to healthy controls.

No differential gene expression for CD4 T cells of MS patients and healthy controls.

Background: Multiple sclerosis-associated genetic variants indicate that the adaptive immune system plays an important role in the risk of developing multiple sclerosis. It is currently not well understood how these multiple sclerosis-associated genetic variants contribute to multiple sclerosis risk. CD4 T cells are suggested to be involved in multiple sclerosis disease processes.

Quantitative proteomic analyses of CD4 and CD8 T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls.

Background: Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. However, T cells play a central role in the pathogenesis by crossing the blood-brain-barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset.

The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells.

Multiple sclerosis (MS) is an inflammatory, demyelinating disorder of the central nervous system that develops in genetically susceptible individuals. The majority of the MS-associated gene variants are located in genetic regions with importance for T-cell differentiation. Vitamin D is a potent immunomodulator, and vitamin D deficiency has been suggested to be associated with increased MS disease susceptibility and activity.

Multiple Sclerosis Risk Allele in CLEC16A Acts as an Expression Quantitative Trait Locus for CLEC16A and SOCS1 in CD4+ T Cells.

For multiple sclerosis, genome wide association studies and follow up studies have identified susceptibility single nucleotide polymorphisms located in or near CLEC16A at chromosome 16p13.13, encompassing among others CIITA, DEXI and SOCS1 in addition to CLEC16A. These genetic variants are located in intronic or intergenic regions and display strong linkage disequilibrium with each other, complicating the understanding of their functional contribution and the identification of the direct causal variant(s).

National survey of potential heart beating solid organ donors in Sweden.

Sweden has about 135 heart beating solid organ donors per year among 9.2 million inhabitants. Earlier estimations have suggested that 250-300 of potential heart beating donors might be available in the country annually.

[The shipwreck of "Der Brandtaucher" in the then territory of the Danish monarchy - in Kiel's harbor - February 1, 1851].

This article describes the first German submarine - "Der Brandtaucher" - constructed in Schleswig-Holstein by Wilhelm V. Bauer in 1850. The submarine was built as a special weapon to attack the Danish blokading warships of the harbour of Kiel during the First Danish-German War 1848-51.

["The case at Eckernförde" April 5, 1849].

The article describes the medical aspects on board the danish naval vessels "Gefion", "Chr. den VIII", "Geiser" and "Hekla" as well as other assignments which demanded special abilities of the naval doctors and the head doctor at "Christians Plejehjem" under and after the sea battle at Eckernförde the 5th of April 1849. The conditions for the wounded on board "Gefion" is described - the cabins, the pharmacy etc.