Publications by authors named "Brittany Hazzard"
Article Synopsis
- - The study investigates how multiple strains of the parasite Plasmodium vivax affect infection in Saimiri monkeys through single cell RNA sequencing and genotyping.
- - Results show that simultaneous infection with two strains can lead to sustained polyclonal infections, but there's no observable difference in how these strains behave in terms of regulation or sexual differentiation.
- - While all parasite genotypes can be transmitted to mosquitoes, not all are successful in causing blood infections, indicating a significant limitation during their early development stages.
Article Synopsis
- Infections often involve multiple genetically distinct strains of parasites, but how this affects the parasites' development and transmission is not well understood.
- A study of monkeys revealed that while simultaneous infections with two strains resulted in sustained polyclonal infections, there were no significant differences in parasite regulation or sexual differentiation.
- The research also indicated that although all parasite genotypes can be transmitted to mosquitoes, not all contribute equally to blood infections later, suggesting a critical selection process during early development.
Plasmodium vivax infections often consist of heterogenous populations of parasites at different developmental stages and with distinct transcriptional profiles, which complicates gene expression analyses. The advent of single cell RNA sequencing (scRNA-seq) enabled disentangling this complexity and has provided robust and stage-specific characterization of Plasmodium gene expression. However, scRNA-seq information is typically derived from the end of each mRNA molecule (usually the 3'-end) and therefore fails to capture the diversity in transcript isoforms documented in bulk RNA-seq data.
View Article and Find Full Text PDFPre-hospital treatment of traumatic brain injury (TBI) with co-existing polytrauma is complicated by requirements for intravenous fluid volume vs. hypotensive resuscitation. A low volume, small particle-size-oxygen-carrier perfluorocarbon emulsion NVX-428 (dodecafluoropentane emulsion; 2% / could improve brain tissue with minimal additional fluid volume.
View Article and Find Full Text PDFBackground: Aeromedical evacuation to definitive care is standard in current military conflicts. However, there is minimal knowledge on the effects of hypobaria (HYPO) on either the flight crew or patients. The effects of HYPO were investigated using healthy swine.
View Article and Find Full Text PDFBackground: There is inadequate information on the physiologic effects of aeromedical evacuation on wounded war fighters with traumatic brain injury (TBI). At altitudes of 8,000 ft, the inspired oxygen is lower than standard sea level values. In troops experiencing TBI, this reduced oxygen may worsen or cause secondary brain injury.
View Article and Find Full Text PDFBackground: Hypoxia is a critical secondary injury mechanism in traumatic brain injury (TBI), and early intervention to alleviate post-TBI hypoxia may be beneficial. NVX-108, a dodecafluoropentane perfluorocarbon, was screened for its ability to increase brain tissue oxygen tension (PbtO2) when administered soon after TBI.
Methods: Ketamine-acepromazine anesthetized rats ventilated with 40% oxygen underwent moderate controlled cortical impact (CCI)-TBI at time 0 (T0).