Thraustochytrids are emerging as a valuable biomass source for high-quality omega-3 polyunsaturated fatty acids (PUFAs), crucial for both human and animal nutrition. This research focuses on cultivating SR21 using cost-effective agro-industrial by-products, namely sugarcane molasses (SCM), corn steep liquor (CSL), and residual yeast cream (RYC), to optimize biomass and lipid production through a comprehensive multistep bioprocess. The study involved optimization experiments in shake flasks and stirred-tank bioreactors, where we evaluated biomass, lipid content, and DHA yields.
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February 2022
Traditionally, lipid-producing microorganisms have been obtained via conventional bioprospecting based on isolation and screening techniques, demanding time and effort. Thus, high-throughput sequencing combined with conventional microbiological approaches has emerged as an advanced and rapid strategy for recovering novel oleaginous microorganisms from target environments. This review highlights recent developments in lipid-producing microorganism bioprospecting, following (i) from traditional cultivation techniques to state-of-the-art metagenomics approaches; (ii) related topics on workflow, next-generation sequencing platforms, and knowledge bioinformatics; and (iii) biotechnological potential of the production of docosahexaenoic acid (DHA) by Aurantiochytrium limacinum, arachidonic acid (ARA) by Mortierella alpina and biodiesel by Rhodosporidium toruloides.
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August 2021
Omega-3 produced by marine thraustochytrids has appeared as an alternative to fish oil and an eco-friendly solution to overfishing. Herein, an integrative analysis of metagenetics and high-throughput screening was used for bioprospecting marine thraustochytrids from southern Brazil mangrove and coastal seawater. All sampled environments showed biodiversity and abundance of SAR clade.
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August 2019
Oil palm empty fruit bunches is a lignocellulosic feedstock with biotechnological potential and thousands of tons are generated in the world each year. Filamentous fungi producing xylanases and ligninases in biopulping to obtain cellulose is a pulp improvement alternative. The enzymatic cocktail was produced in solid-state biopulping by Aspergillus sp.
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