Publications by authors named "Brigida T L Lucena"

The open process used to ferment sugar cane juice or molasses to produce ethanol fuel is prone to contamination by bacterial cells of different species, in particular Lactobacilli. The situation can be exacerbated by the emergence of resistant cells to industrial antibiotics that are normally used to combat this contamination. In this work, two Lactobacillus vini isolates from ethanol distilleries were identified and found to be resistant to doxycycline, a tetracycline derivative, although sensitive to other antibiotics tested.

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Four strains of lactic acid bacteria isolated from cachaça and alcohol fermentation vats in Brazil were characterised in order to determine their taxonomic position. Phylogenetic analysis revealed that they belong to the genus Oenococcus and should be distinguished from their closest neighbours. The 16S rRNA gene sequence similarity against the type strains of the other two species of the genus was below 94.

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This work describes the effects of the presence of the yeast Dekkera bruxellensis and the bacterium Lactobacillus vini on the industrial production of ethanol from sugarcane fermentation. Both contaminants were quantified in industrial samples, and their presence was correlated to a decrease in ethanol concentration and accumulation of sugar. Then, laboratory mixed-cell fermentations were carried out to evaluate the effects of these presumed contaminants on the viability of Saccharomyces cerevisiae and the overall ethanol yield.

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Article Synopsis
  • This study is the first to quantify and identify Lactic Acid Bacteria (LAB) in Brazilian bioethanol production, highlighting their potential to compete with yeast for nutrients.
  • A total of 489 LAB isolates were collected from four distilleries, with LAB concentrations ranging significantly in fermentation tanks and crude sugar cane juice.
  • The research found high diversity among LAB species, primarily Lactobacillus, with some strains exhibiting tolerance to high ethanol levels, indicating their possible role in affecting bioethanol efficiency.
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