Bioactivity of fractions and constituents of Piper capense fruits towards a broad panel of cancer cells.

Ethnopharmacological Relevance: Piper capense is a medicinal spice whose fruits are traditionally used as aqueous decoction to heal several ailments such as trypanosomiasis, helminthic infections, and cancer.

Aim Of The Study: (1) To perform phytochemical investigation of the methanol extract of Piper capense; (2) to evaluate the cytotoxicity of botanicals (PCF, fractions PCFa-e), isolated phytochemicals on a broad panel of animal and human cancer cell lines; (3) to evaluate the induction of apoptosis of the most active samples.

Material And Methods: Resazurin reduction assay (RRA) was used to determine the cytotoxicity of the studied samples.

Bark extract of Cassia sieberiana DC. (Caesalpiniaceae) displayed good antibacterial activity against MDR gram-negative phenotypes in the presence of phenylalanine-arginine β-naphthylamide.

Background: Multidrug-resistant (MDR) bacteria remain a major cause of morbidity and mortality globally. The present study was designed to investigate the in vitro antibacterial activities of crude methanol extract and constituents isolated by Column Chromatography (CC) from Cassia sieberiana bark (CSB) against ten MDR Gram-negative bacteria, as well as the mechanisms of action of the most active sample.

Methods: The antibacterial activity of the tested samples (extract, the fractions and their compounds isolated by CC and the structures obtained by exploiting H and C Nuclear magnetic resonance (NMR) spectra) in the presence and absence of an efflux pumps inhibitor, phenylalanine-arginine β-naphthylamide (PAβN), was evaluated using the micro-dilution method.

Caffeate and piperidine-3-ol derivatives from the stem bark of .

A new caffeate derivative from the ethanol extract of the stem bark of DC. is described herein along with the known secondary metabolites spectaline (), -6-cassine (), 3--methyl--inositol (), monobehenin (), octyl nonadecyloate (), -sitosterol (), stigmasterol () and sitosterol 3---D-glucopyranoside (). The chemical structures were elucidated by means of various spectroscopic and spectrometric techniques.