Publications by authors named "Brianna Close"
Article Synopsis
- * Vero E6 cells, although commonly used, show limitations in growing new viral variants effectively, leading researchers to explore alternative human cell lines.
- * The Caco-2/AT and HuH-6/AT cell lines were identified as highly effective for SARS-CoV-2 propagation, outperforming Vero E6, and enabling the generation of genetically reliable recombinant viruses.
The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in more than 235 million cases worldwide and 4.8 million deaths (October 2021), with various incidences and mortalities among regions/ethnicities. The coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63 utilize the angiotensin-converting enzyme 2 (ACE2) as the receptor to enter cells.
View Article and Find Full Text PDFSARS-CoV-2 can infect multiple organs, including lung, intestine, kidney, heart, liver, and brain. The molecular details of how the virus navigates through diverse cellular environments and establishes replication are poorly defined. Here, we generated a panel of phenotypically diverse, SARS-CoV-2-infectible human cell lines representing different body organs and performed longitudinal survey of cellular proteins and pathways broadly affected by the virus.
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- * The coronavirus 3C-like protease (3CLpro) is a key target for antiviral drug development, as it is essential for the virus's replication process.
- * A new luminescence-based biosensor assay has been developed to test small molecule inhibitors of the SARS-CoV-2 3CLpro, along with a rabbit antiserum that can identify both SARS-CoV and SARS-CoV-2 proteases, aiding in pre-clinical testing of potential treatments.
SARS-CoV-2 can infect multiple organs, including lung, intestine, kidney, heart, liver, and brain. The molecular details of how the virus navigates through diverse cellular environments and establishes replication are poorly defined. Here, we performed global proteomic analysis of the virus-host interface in a newly established panel of phenotypically diverse, SARS-CoV-2-infectable human cell lines representing different body organs.
View Article and Find Full Text PDFCOVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for the prevention or treatment to curtail mortality and morbidity. No vaccine to boost mucosal immunity, or as a therapeutic, has yet been developed to SARS-CoV-2. In this study, we discover and characterize a cross-reactive human IgA monoclonal antibody, MAb362.
View Article and Find Full Text PDFCOVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for the prevention or treatment to curtail mortality and morbidity. No vaccine to boost mucosal immunity or as a therapeutic has yet been developed to SARS-CoV-2. In this study we discover and characterize a cross-reactive human IgA monoclonal antibody, MAb362.
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