Bliss procedure for undescended ovaries.

Objective: To highlight a novel surgical approach for the management of undescended ovaries in those presenting with infertility, to allow for potential transvaginal egg retrieval. The video demonstrates a novel surgical approach for mobilization and oophoropexy of undescended ovaries to allow for future transvaginal egg retrieval in the context of artificial reproductive technology (ART).

Design: Case report.

Sydney contained in bag morcellation for laparoscopic myomectomy.

Study Objective: To demonstrate a new technique of contained in bag morcellation of a myoma after laparoscopic myomectomy.

Design: Step-by-step explanation of the technique in a narrated video.

Intervention: Contained In Bag Morcellation of myoma after laparoscopic myomectomy.

The Sydney Contained In Bag Morcellation technique.

Study Objective: To demonstrate a modification of the Shibley single-port technique suitable for morcellation of large myomatous uteri after total laparoscopic hysterectomy in a contained environment within the abdominal cavity [1].

Design: Step-by-step explanation of the technique using descriptive text and an educational video.

Setting: In light of recent concern about the use of power morcellators and increasing the risk of disseminating occult leiomyosarcomatous myoma fragments throughout the abdominal cavity, we propose this new technique for management of morcellation of large myomatous uteri after total laparoscopic hysterectomy, to contain the morcellation process and minimize the risk.

Early treatment with alglucosidase alpha prolongs long-term survival of infants with Pompe disease.

In a previous 52-wk trial, treatment with alglucosidase alpha markedly improved cardiomyopathy, ventilatory function, and overall survival among 18 children <7 mo old with infantile-onset Pompe disease. Sixteen of the 18 patients enrolled in an extension study, where they continued to receive alglucosidase alpha at either 20 mg/kg biweekly (n = 8) or 40 mg/kg biweekly (n = 8), for up to a total of 3 y. These children continued to exhibit the benefits of alglucosidase alpha at the age of 36 mo.

"What do kids know": a survey of 420 Grade 5 students in Cambodia on their knowledge of burn prevention and first-aid treatment.

Introduction: Cambodia is a developing country of 13 million people where there are an estimated 20,000 burns and 2000 burn deaths annually. Two thirds of the burns occur to children under the age of 10 years. The purpose of this study was to determine the knowledge of burn prevention and first aid for burns in Grade 5 Cambodian school children, as baseline information to design a burn prevention campaign.

A novel element regulates expression of the proximal human proglucagon promoter in islet cells.

The human and rat proglucagon gene proximal promoter regions have differing transcriptional activities in pancreatic islet cell lines, with 300 bases of rat proglucagon 5' flanking sequence being sufficient to support expression in rodent islet cell lines, while the homologous human sequences are transcriptionally silent. To better understand the changes in promoter activity between human and rat we have used a comparative approach and cloned promoters from diverse mammalian species and tested their transcriptional activities. Proglucagon gene proximal promoter regions from species representing three orders of mammals (rodents, artiodactyls, and carnivores) support transcription in rodent islet cell lines, while promoters from primates (human and rhesus monkey), despite significant sequence conservation, failed to drive reporter gene expression.

Quantification of human tissue kallikreins in the stratum corneum: dependence on age and gender.

Human tissue kallikreins are a family of 15 trypsin or chymotrypsin-like secreted serine proteases (hK1-hK15). hK5, hK6, hK7, hK8, and hK13 have been identified in the stratum corneum (SC), stratum granulosum, and skin appendages. It has been reported that hK5 and hK7 degrade desmosomes/corneodesmosomes, suggesting that kallikreins are responsible for desquamation.