Competitive assembly resolves the stoichiometry of essential proteins in infectious HIV-1 virions.

Unlabelled: During assembly on the plasma membrane, HIV-1 virions incorporate Gag-Pol as well as gp120/gp41 trimers. The Pol region consists of protease, reverse transcriptase and integrase precursors which are essential enzymes required for maturation, reverse transcription, and integration of the viral genome in the next host. gp120/gp41 trimers catalyze the fusion of the virion with its next host.

Gag-Gag Interactions Are Insufficient to Fully Stabilize and Order the Immature HIV Gag Lattice.

Immature HIV virions harbor a lattice of Gag molecules with significant ordering in CA-NTD, CA-CTD and SP1 regions. This ordering plays a major role during HIV maturation. To test the condition in which the Gag lattice forms in vivo, we assembled virus like particles (VLPs) by expressing only HIV Gag in mammalian cells.

Trauma in Pregnancy: A Comprehensive Approach to the Mother and Fetus.

Trauma is a significant contributor to maternal and fetal morbidity and death in the United States. The nature of the evaluation of the pregnant trauma patient is intense and multidisciplinary. Although it invites unique opportunities for collaboration with our surgical colleagues, it also increases the risk of misunderstanding and conflict.