Publications by authors named "Brian Japp"
Methods Mol Biol
May 2012
Article Synopsis
- Accurate measurement of cytokine concentrations is crucial for studying inflammation, and ELISA (enzyme-linked immunosorbent assay) is an effective method for this purpose due to its affordability and precision.
- The text outlines a step-by-step process for creating an indirect sandwich ELISA to effectively detect and quantify cytokines and other biomarkers.
- It also addresses the use of sequential ELISA assays for analyzing multiple cytokines from small sample volumes and introduces the idea of multiplex ELISA, discussing its cost and time implications.
