Publications by authors named "Brian J Watson"
Article Synopsis
- A study investigated how four different endoglucanases interact with amorphous cellulose films using neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D).
- The endoglucanases examined included two from fungi and two from thermophilic bacteria, highlighting the potential of ionic liquid pretreatment in disrupting cellulose's crystalline structure.
- Results showed that the presence of carbohydrate-binding modules (CBMs) in Cel45A and Cel5H allowed them to penetrate and digest the cellulose films more effectively than Cel9A and Cel5A, which lack CBMs, indicating varied mechanisms of action among the enzymes.
Saccharophagus degradans 2-40 is a marine gamma proteobacterium that can produce polyhydroxyalkanoates from lignocellulosic biomass using a complex cellulolytic system. This bacterium has been annotated to express three surface-associated β-glucosidases (Bgl3C, Ced3A, and Ced3B), two cytoplasmic β-glucosidases (Bgl1A and Bgl1B), and unusual for an aerobic bacterium, two cytoplasmic cellobiose/cellodextrin phosphorylases (Cep94A and Cep94B). Expression of the genes for each of the above enzymes was induced when cells were transferred into a medium containing Avicel as the major carbon source except for Bgl1B.
View Article and Find Full Text PDFBacteria and fungi are thought to degrade cellulose through the activity of either a complexed or a noncomplexed cellulolytic system composed of endoglucanases and cellobiohydrolases. The marine bacterium Saccharophagus degradans 2-40 produces a multicomponent cellulolytic system that is unusual in its abundance of GH5-containing endoglucanases. Secreted enzymes of this bacterium release high levels of cellobiose from cellulosic materials.
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