H O Release Assay.

Reactive oxygen species are ubiquitous in nature, and function as signalling molecules in biological systems; they may also contribute to oxidative stress in several pathobiological disease states. In this report, we describe a simple, reliable, sensitive, and specific assay for the detection and quantitation of hydrogen peroxide (H O ) release by living cells, organoids, or tissues. Furthermore, the low cost of reagents required for this assay makes it inexpensive relative to commercial kits.

The REGγ-Proteasome Regulates Spermatogenesis Partially by P53-PLZF Signaling.

Development of spermatogonia and spermatocytes are the critical steps of spermatogenesis, impacting on male fertility. Investigation of the related regulators benefits the understanding of male reproduction. The proteasome system has been reported to regulate spermatogenesis, but the mechanisms and key contributing factors in vivo are poorly explored.